Effects Of Heme Oxygenase-1 Shorthairpin RNA Transfection On Biological Behaviors Of Gastric Cancer Line SGC7901

Objective:Gastric carcinoma is one of the most Commonly Gastrointestinal neoplasm in our country and still increasing year by year,which badly do harm to people's health.It is of significant meaning to understand the pathogenesis and metastatic patterns of gastric carcinoma.Heme oxygenase-1(HO-1),also called Hot Shock Protein 32(HSP32) is the start enzyme and the key enzyme of resolving heme,that is released by senescent red blood cell and hemoglobin in hemocirculatory,into Iron Ions,biliverdin and carbon monoxide.The three kind of products are the main effect molecule of HO-1 in anti-inflammation,anti-oxadation, anti-Apoptosis and anti-hyperplasia.In this study,our purpose is to investigate the effects of heme oxygenase-1 short hairpin RNA transfection on biological behaviors of gastric cancer lineSGC7901.Methods:A eukaryotic expression plasmid of shRNA targeting on HO-1 was constructed and was transiently transfected into human gastric cancer line SGC7901. Expression of HO-1mRNA and protein were detected by reverse transcription polymerase chain reaction(RT-PCR) and immunocytochemical stainning.Cell proliferation and cell cycle were determined by MTT and flow cytometry assay.The experimental data were expressed with((?)±s) and analysed by SAS 9.13 statistic software.Results:HO-1 shRNA effectively inhibited the expression of HO-1mRNA (62.4%)and protein(67.6%),cell growth[2.036±0.072 vs 2.783±0.067(72h),P＜0.05],and decreased the cells in G₀/G₁ phase(52.025±1.638vs67.525±1.938,P＜0.05).Conclusions:HO-1 shRNA effectively inhibited the expression of HO-1,thus inhibiting the growth of the tumor cells.