The Clinical Significance Of P-glycoprotein And Lung Resistance Protein Expressions In Infiltrating Breast Carcinoma Tissues

Objective:Primary breast carcinoma is one of the most common malignances.Chemotherapy is one of important part of breast carcinoma' therapy.It has been found that the multidrug-resistance(MDR) is the main reason that leads to failure of chemotherapy.P-gp,LRP have been proposed as correlation with multidrug-resistance.In this study,our aim is to understand the expression and significance of P-gp,LRP in breast carcinoma and the relationship of between the two drugresistance-associated proteins.Materials and Methods:We collected 40 specimens of breast carcinoma of Shandong provincal Qianfoshan hospital during the period from July 2007 to September 2009.Single-cell suspension with intact cellular membrane taken from operative specimens of breast carcinoma were prepared using rubbing-net technique. Flow Cytometry(FCM) was used to examine the expression of proteins P-gp and LRP in carcinoma tissues and corresponding para-carcinoma tissues.Results:1.The expressions of P-gp and LRP in carcinoma tissues and corresponding para-carcinoma tissues:In breast carcinoma their expression level of P-gp was(23.3±13.7)%.In corresponding para-carcinoma tissues,their expression level of P-gp was(5.1±5.0)%.The expression of P-gp was higher in breast carcinoma tissues than that in corresponding para-carcinoma tissues(p＜0.05).In breast carcinoma their expression level of LRP was(35.4±17.8)%.In corresponding para-carcinoma tissues,their expression level of LRP was(6.8±6.5)%.Their differences were obvious(p＜0.05).2.The expression of P-gp and and clinical-pathological data:There were not notable differences of two drug resistance related protein expressions in different ages,different tumor sizes,different pathological types,different clinical stages,estrogen receptor or progestin receptor negative and positive patients(p＞0.05).There was no notable difference of P-gp expression in lymph node metastasis negative and positive patients(p＞0.05).The expression of LRP was higher in lymph node metastasis positive patients than that in negative patients(p＜0.05).The expression of LRP was significantly associated with axillary node postive.3.The correlation among the expression of P-gp and LRP:The Pearson correlation analysis showed that there was a positive correlaion between the expression of P-gp and the expression of LRP(p＜0.05)Conclusions:1.The expressions of P-gp and LRP in breast carcinoma were different. 2.The expression of LRP in breast carcinoma was significantly associated with axillary node postive,which perhaps has a close relationship with metastasis of breast carcinoma.It can become a target in forecasting breast carcinoma prognosis.3.The co-expression of P-gp and LRP can be found in the primary drug resisitance of breast carcinoma.Measuring the expression of P-gp and LRP in breast carcinoma together might guide the choice of chemotherapy drugs.