| Background:Central nervous system's diseases and the damages have been the significant threats for human health,and are also the focal point and the difficulties which the medicine worker studies.Use the embryo nerve stem cells transplantation to treat nervous system diseases or the damages has made the significant developments,and has been applied in Parkinson,stroke as well as the skull wound treatment and obtained the remarkable curative effects.But the nerve stem cells have many shortcomings such as the origin lack,the immunity to repel as well as to violate ethics.In order to avoid these limitations,seek the effective new seed cells as the substitution of the embryo nerve stem cell(NSCs) has became a new focal point in recent research works.Bone marrow mesenchymal stem cells(MSCs) are a kind of non- hematopoietic stem cells which has become a hotspot in medical field because of its biological effects.Recently it has been found that bone marrow MSCs have multiple differentiation potential,more importantly,its differentiation has tissue specificity. That is to say,the tissue microenvironment which they arrived can induce directional differentiations.This discovery has important significance to stem cell transplantation and cell replacement therapy.MSCs are not only easy to gain,to separate,to raise,to increase the purification,but also can maintain merits after several generation in vitro. MSCs still have a character to induce immune tolerance and immune suppression. What is more,MSCs has the protective function to the central nervous system. Therefore,marrow MSCs has been widely applied in stem cells transplantation, organization project and organ transplanting immunity treatment,has brought the hopes for many kinds of diseases' treatments.The carbon monoxide poisoning(COP) is also called the gas poisoning,occurs in airtight spaces which are filled with highly concentrated CO.The acute poisoning causes the damages to the whole body tissue,especially serious to the cerebral cortex and the cornu ammonis.Many acute poisoning patients may have the serious delayed neuropsychiatric sequelae(DNPS).The poisoning causes the brain damages which have reduced patients' life quality greatly,brings the huge pain to the patients.We mainly use high pressure oxygen to treat acute poisoning,other medicines for auxiliary therapy.But the results are not as good as we want.So we attempt to use the stem cell transplantation to treat the damages in brain caused by the carbon monoxide poisoning.Objective:Investigate the effect and mechanism of mesenchymal stem cells (MSCs) transplanted for treatment of brain damage caused by carbon monoxide poisoning(COP) in rats.Methods:MSCs are isolated from the bone marrow of Wistar rats,cultured and generated in vitro and then prepare MSCs to the suspension.The rats carbon monoxide poisoning models are established by peritoneal injection according to 100～150mL/kg.MSCs which marked with PKH26 are transferred into rats through internal carotid artery.And use the NIHSS and Morris water labyrinth experiment to examine the nervous system function of model rats.Detect the damage area cells in cornu ammonis with immunohistochemical methods and Nissl's staining.Results:MSCs can survive and reduce apoptosis of nerve cells.One and four week later,Morris water labyrinth experiments demonstrate that the rats' space learning capability has improved greatly and the brain organization pathological changes have been reduced obviously.(P＜0.05)Conclusion:We take the good stem cell raise method in vitro.We receive the good curative effect by using mesenchymal stem cells(MSCs) transplantation to treat the brain damage cause by carbon monoxide poisoning(COP) in rats.
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