The Pharmaceutical Studies On The Ginsenoside Rg3 Microemulsion For Oral Administration

Ginsenoside Rg3 is a kind of rarity ginsenoside in red ginseng,coming from panoxadiol drawing redients off the glycosyl at C-20 when processing red ginseng.It has the pharmacological effects of antitumor.But it's very low bioavailability limited full use of clinical therapeutic effect for oral administration.To improve bioavailability,ginsenoside Rg3 microemulsion was prepared based on the theories and methods of physical chemistry and pharmaceutics.The quality of ginsenoside Rg3 microemulsion was assessed by transmission electron microscope,photon correlation spectrometer,HPLC and so on.This study will lay a good foundation for it's further study.The method of producing ginsenoside Rg3 by acidolysis is studied.Through investigating influencing factor in process of acidolysis,the best produce technology is determined.The quantitative method for ginsenoside Rg3 by HPLC is established,and the and the foundation of formula is studied.Further more,based on the ternary phase diagram,choosing the microemulsion region area as index,the best microemulsion system (TritonX-100/Isopropanol/Ethyl oleate/Water ) is determined.The quantitative method for ginsenoside Rg3 in its microemulsion by HPLC is also established.The formula is optimized based on the drug loading of microemulsion and its stability.The blank microemulsion formula is determined.(30%ritonX-100,15%isopropanol, 5%ethyl oleate,50%water,g/g ).The contant of saturated loading on drug microemulsion is 3.51±0.68,3.47±0.45,3.52±0.71mg/mL.O/W ginsenoside Rg3 microemulsion was obtained by Staining and electric conductance.The average particle diameter was 43.5nm;viewing under the electron microscope. It's shape was spheroidal.The size distribution was among 10～100nm;The result of dissolution experiment showed that the releasing speed and level of ginsenoside Rg3 microemulsion were greater than common capsule sold on the market in two mediators with different pH.Stability investigation showed that ginsenoside Rg3 microemulsion had a good stability.