| Angelica is the dried root of Angelica sinensis (oliv) Diels, and is common clinical drug. The Angelica growing in Min county of Gansu province that is to be considered as the best one in Angelicas. It is sweet and pungent in taste, and warm in nature, acts on the liver, heart and spleen meridians and has the effects of invigorating the blood, promoting blood circulation, regulating menstruation, alleviating pain, moistening the bowels, and relieving constipation. Modern pharmacology further studies proved that angelica has the effects of promoting blood circulation by removing blood stasis, invigorating the blood and alleviating pain. It was also found that angelica had the effects of anti-inflammation, scavenging the free radical, protecting liver, cholagogue, protecting kidney and enhancing immune function. There were different processing methods and different processed products in Traditional Chinese Medical (Veterinary) history. At the present time, some common processed products of angelica such as oil processed angelica,dust processed angelica,wine processed angelica and so on. The study chose·OH , O2-., mouse liver homogenates spontaneous lipid peroxidation, erythrocytic membrane lipid peroxidation and erythrocyte hemolysis induced by H2O2 in vitro to evaluate the oxidation resistance of different processed products of angelica; to research the difference between different processed products of angelica.1. The effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) were tested on cleaning·OH free radical by producing hydroxyl with Fenton reaction. Experimental results suggested that water and alcoholic solutions from different processed products of angelica(raw,oil and wine) had significant antioxidative actions on·OH free radical in 5～80 mg/mL and 20～160 mg/mL respectively. All showed a significant dose-effective relationship, the order of·OH scavenging action of water solutions from different processed products of angelica(raw,oil and wine) is: wine processed angelica>oil processed angelica>raw processed angelica. The order of·OH scavenging action of alcoholic solutions from different processed products of angelica(raw,oil and wine) is: wine processed angelica>raw processed angelica>oil processed angelica. The scavenging effect of water extracts were superior to alcoholic extracts from angelica in the same concentration.2. The effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) were tested on cleaning O2-. free radical with the autoxidation of pyrogallol model respectively. Experimental results suggested that water and alcoholic extracts from different processed products of angelica(raw,oil and wine) had significant effect on scavenging O2-. free radical in 25～200 mg/mL. The order of O2-. scavenging action of water solutions from different processed products of angelica(raw,oil and wine) is: wine processed angelica>raw processed angelica>oil processed angelica. The order of O2-. scavenging action of alcoholic solutions from different processed products of angelica(raw,oil and wine) is: wine processed angelica>raw processed angelica>oil processed angelica. The scavenging effect of water extracts were superior to alcoholic extracts from different processed products of angelica(raw and wine); the scavenging effect of alcoholic extracts were superior to water extracts from oil processed products of angelica.3. The effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) were tested on MDA spontaneous formation in liver homogenates of mice by TBA colorimetry. Experimental results suggested that water and alcoholic extracts from different processed products of angelica(raw,oil and wine) can significantly inhibited MDA spontaneous formation in homogenates of mice liver in 25～200 mg/mL, Inhibitory effect increases gradually with increasing of drug concertration, inhibitory rate showed a concentration-dependant manner. The order of antioxidation of water solutions from different processed products of angelica(raw,oil and wine) is: wine processed angelica>raw processed angelica>oil processed angelica. The order of antioxidation of alcoholic solutions from different processed products of angelica(raw,oil and wine) is: raw processed angelica>wine processed angelica>oil processed angelica. The inhibitory effect of alcoholic extracts from different processed products of angelica(raw,oil and wine) were better than water extracts from angelica in the same concentration.4. The effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) on erythrocytic membranous lipid peroxidatic reaction that induced by H2O2 was tested by spectrophotometer. Experimental results suggested that water solutions from different processed products of angelica(raw,oil and wine) can significantly inhibit erythrocytic membranous lipid peroxidatic reaction in 50～400 mg/mL;alcoholic solutions from different processed products of angelica(raw,oil and wine) can significantly inhibit erythrocytic membranous lipid peroxidatic reaction in 12.5～100 mg/mL. Inhibitory effect increases gradually with increasing of drug concentration, inhibitory effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) showed a significant dose-effective relationship. The order of antioxidation of water solutions from different processed products of angelica(raw,oil and wine) is: raw processed angelica>oil processed angelica>wine processed angelica.the order of antioxidation of alcoholic solutions from different processed products of angelica(raw,oil and wine) is: raw processed angelica>wine processed angelica>oil processed angelica. The inhibitory effect of alcoholic extract from different processed products of angelica(raw,oil and wine) for erythrocytic membranous lipid peroxidatic reaction that induced by H2O2 were superior to water extract from different processed products of angelica(raw,oil and wine) in the same concentration.5. The effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) on erythrocytic membranous hemolysis that induced by H2O2 was tested by spectrophotometer. Experimental results suggested that water solutions from different processed products of angelica(raw,oil and wine) can significantly inhibit erythrocytic membranous hemolysis in 25～200 mg/mL; alcoholic solutions from different processed products of angelica(raw,oil and wine) can significantly inhibit erythrocytic membranous hemolysis in 50～400 mg/mL. Inhibitory effect increases gradually with increasing of drug concentration, inhibitory effect of water and alcoholic solutions from different processed products of angelica(raw,oil and wine) showed a significant dose-effective relationship. The order of antioxidation of water solutions from different processed products of angelica(raw,oil and wine) is: wine processed angelica>oil processed angelica>raw processed angelica. The order of antioxidation of alcoholic solutions from different processed products of angelica(raw,oil and wine) is: oil processed angelica>raw processed angelica>wine processed angelica. The inhibitory effect of water extracts from different processed products of angelica(raw,oil and wine) for erythrocytic membranous hemolysis that induced by H2O2 were superior to alcoholic extracts from different processed products of angelica(raw,oil and wine) in the same concentration.Conclusion : The different processed products of angelica(raw,oil and wine) possess complicated ingredients, It is not reasonable to assess oxidation resistance of different processed products of angelica(raw,oil and wine) using one or more ingredients. The study choose water and alcoholic solutions from different processed products of angelica(raw,oil and wine) to research the oxidation resistance of different processed products of angelica(raw,oil and wine), In order to in the round and objectively assess oxidation resistance of different processed products of angelica(raw,oil and wine). Different processing methods and extracting mediums are two important factors for angelica in free radical scavenging and oxidation resistance; Wine processed angelica is the best one of three in free radical scavenging and oxidation resistance. Comparatively speaking, alcoholic extracts are superior to water extracts from different processed products of angelica(raw,oil and wine). It is rational tochoose cleaning O2-. free radical index and MDA spontaneous formation in liver homogenates of mice by TBA colorimetry index to assess oxidation resistance of different processed products of angelica(raw,oil and wine).
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