Expression And Clinical Significance Of Bmi-1 Protein And HTERT Protein In Non-small Cell Lung Cancer

Objective:The aim of this study was to explore the expression of Bmi-1 and hTERT in non-small cell lung cancer,and to analysis their correlation with clinical pathological parameters and the relationship between Bmi-1 and hTERT,to seek their clinicopathologic significance in nonsmall cell lung cancer.Methods:The expression of Bmi-1 protein and hTERT protein was examined in 65 cases of non-small cell lung cancer and 20 cases of normal lung tissues by immunohistochemistry SP methods.The data was analyzed by SPSS15.0,x~2-test and Spearman'srank correlation analysis were used to analyze the difference between group,a=0.05 was considered as satistically significant value.Results:(1) The abnormal expression rates of Bmi-1 in non-small cell lung cancer were 61.5%(40/65) and 30%(6/20) in normal lung tissues.There was a significant difference between the t-umor tissues and normal lung tissues (P＜0.05).The abnormal expression rates of hTERT in non-small cell lung cancer were 78.5%(51/65) and 25%(5/20) in normal lung tissues.There was a significant difference between the tumor tissues and normal lung tissues (P＜0.05).(2) The abnormal expression of Bmi-1 in lung cancer with lymph node metastasis was 71.1%(32/45).In lung cancer without lymph node metastasis, the abnormal expression rate of Bmi-1 was 40%(8/20).There was a signifi- cant difference between them(P＜0.05).The abnormal expression of Bmi-1 increased with the TNM stage increased.In the periodⅠ～Ⅳtumor tissue,the abnormal expression rates were 25%(3/12),66.7%(14/21),69.2%(18/26),83.3%(5/6) and there were significant difference between them(P＜0.05).In addition,the abnormal expression of Bmi-1 also increased with tumor size increases and it is a significant difference(P＜0.05).The expression of Bmi-1 level was not correlated with gender,age,or tumor pathological types(P＞0.05).The abnormal expression of hTERT in lung cancer with lymph node metastasis was 88.9%(40/45).In lung cancer without lymphnode metastasis, the abnormal expression rate of hTERT was 55%(11/20).There was a significant difference between them(P＜0.05).The abnormal expression of hTERT increased with the TNM stage increased.In the periodⅠ～Ⅳtumor tissue,the abnormal expression rates were 41.7%(5/12),85.7%(18/21),88.5%(23/26),83.3%(5/6)and there were significant difference between them(P＜0.05).In addition,the abnormal expression of hTERT also in-creased with tumor size increases and there was a significant difference(P＜0.05).The expression of hTERT level was not correlated with gender,age,or tumor pathological types(P＞0.05).(3)Relationship between Bmi-1 and hTERT expression in non-small cell lung cancer:Spearman's rank correlation analysis showed a positively correlated with Bmi-1 expression and hTERT expression(r=0.382,p＜0.01).Among 40 cases of Bmi-1 abnormal expression,37 cases of hTERT staining showed abnormal expression,whereas 3 case of hTERT staining showed normal expression.Conclusion:The abnormal expression of Bmi-1 and hTERT in nonsmall cell lung cancer were much higher than normal lung tissue.Bmi-1 expression was positively correlated with hTERT expression in non-small cell lung cancer.The expression of Bmi-1 and hTERT were significant correlated among TNM stage,tumor size and lymph node metastasis.It suggested that Bmi-1 and hTERT acted as an important role in development and metastasis of lung cancer.Co-detected the expression levels of the two proteins might be served as an important factor for determining tumor biological behavior, as well as clinical judgment for prognosis and metastasis.