Preliminary Study On The Murine Reverse Pregnancy Outcome And Pathopoiesis Mechanism With Chlamydia Trachomatis F

Objective1.It is the purpose of establishment the murine model to produce genital infection transvaginally with Chlamydia trachomatis F,and to observe pregnancy outcomes in gestation mice.2.To explore the relationship between the pregnancy outcome of pregnant mouse infected transvaginally with Chlamydia trachomatis F and the levels of Th1 type and Th2 type cytokine, and to explore the affection of the pregnancy outcome and immune status with azithromycin treatment,and to investigate the immunologic mechanism of observe pregnancy outcomes genital infection transvaginally with Chlamydia trachomatis.MethodsAll pregnancy mice were divided to three group randomly,on day 2,3,4 of gestation,the control group A was inoculated intravaginally 30μl of SPG without C. trachomatis F,and the experimental group B was inoculated with 106 C. trachomatis IFU in 30μl of SPG,and the experimental group C was injected azithromycin 10mg/kg (single administration )intraperitoneally on day 8 of gestation after inoculated with 106 C. trachomatis IFU in 30μl of SPG. On day 12 of gestation,the mice were killed and detected the serum cytokine levels of Th1 type ( IFN-γ,TNF-α) / Th2 type( IL-4,IL-10) with euzymelinked immunosorbent assay,and calculated the proportionality of IL-4 /TNF-α,IL-10 /TNF-α. To compare the Differences of the conception rate, the embryo resorption rate,embryo weight, and maternal weight increment with analysis of variance andχ2 test,and To compare the cytokine levels and proportionality of TH1/TH2 among groups with analysis of variance. Results1. The pregnancy failure rate of experiental B group was inoculated intravaginally Chlamydia trachomatis F type strain was 50% it's was significantly higher than control A group that the pregnancy failure rate was 13.3%.The repeated pregnancy failure rate of experiental group B group was 22% that was compared with control group A the repeated pregnancy failure rate was 0, there was a significant difference; the pregnancy failure rate and repeated pregnancy failure rate of experimental group C that was azithromycin intervention group was lower than the group B and higher than the group A,but there was no significant difference of the pregnancy failure rate (P> 0. 05),and there was a significant difference of repeated pregnancy failure rate (P <0. 05).2. there was no significant difference within the three groups about embryo resorption rate, embryo weight,maternal weight incremen.3. Th2-type cytokines (IL-10) of the experiental group inoculated Chlamydia trachomatis was decreased than that of the control group,but Th1-type cytokines (TNF-α) was increased,and the ratio of IL-4 / TNF-α,IL-10 / TNF-αwith the experimental group was significantly higher than the control group.Azithromycin treatment group reduced the failure rate of pregnancy and the level of Th1-type immune bias.Conclusions1.We cultivated Chlamydia trachomatis successfully serotype F,and amplification, sequencing and counting.2. We established animal models successfully with with Chlamydia trachomatis serotype F genital infection transvaginally, and to assess the effection of pregnancy outcome that Chlamydia trachomatis infected pregnant mice and to study the immune status of pregnant mice.Then empirical method was feasible that animal models were established using Chlamydia trachomatis serotype F genital infection transvaginally.3. Chlamydia trachomatis serotype F intravaginal vaccination of pregnant mice can cause pregnancy failure rate and repeated pregnancy failure rate increased, but it was no significant influence of embryo resorption rate,embryo weight,maternal weight incremen.Azithromycin intervention reduced the failure rate of pregnancy, but didn't reduce pregnancy failure rate significantly.4. Chlamydia trachomatis serotype F vaginal inoculation pregnant mice can produce specific cellular and humoral immunity, The cytokines in vivo were drifted toTh1-type , Th1-type cytokines can hinder the development of early embryos, and cause pregnancy loss, and increas the failure rate of pregnancy in mice. Azithromycin intervention can reduce the level of Th1-type drift and reduce the failure rate of pregnancy in mice.