Relationship Between Infections Of CT Or UU And Treatment Outcome Of IVF-ET

Background Chlamydia trachomatis (CT) and Ureaplasma urealyticum (UU)are common pathogens in human genital meatus. It is proved by a lot studies at home and abroad that CT and UU relate to happening and developing of multifold urogenital diseases. As their infections are asymptomatic, it is not easy to perceive them. So their infections can repeatedly relapse and lead to urogenital chronic inflammation, so much as infertility. IVF-ET is a acknowledged effective cure for infertility. The application of IVF-ET can decrease the influence of reproductive organs diseases on fecundity. But the studies whether treatment outcome of IVF-ET is affected by infections of CT and/or UU are less at home.Objective The purpose of this study was to investigate whether infections of CT or UU relate to treatment outcome of IVF-ET, and probe into influence of CT or UU on human fecundity. This study can provide a reference how we can improve success in IVF-ET.Methods Calculated the infertile couples of IVF-ET treatment between November 2004 to September 2005. Detect three specific antibodies against CT of wives serum by Enzyme Linked Immunosorbent assay (ELISA) . And CT-DNA and UU-DNA of wives cervical secretion and husbands'sperm was detected by Polymerase Chain Reaction (PCR). Finally comparison analysis in detected resulte and patients'treatment outcome.Results 1. The positive rate of specific antibodies against CT in no pregnancy group was higher than one in pregnancy group, but having no significant difference(P>0.05). 2. The percent of the antibodies compositive type of + + + had a significant difference in pregnancy group and no pregnancy group. This type just occured in no pregnancy group. Neither of groups had two Types of - - + and + - + . 3. The positive rate of CT-DNA and UU-DNA detected by PCR had no difference in two groups. 4. The positive rate of CT-PCR is obvious less than that of specific antibodies of the IgM class against CT .Conclusion 1. From experimental results, the infections of CT and UU have no significant effect on the treatment outcome of the infertile patients of IVF-ET treatment, but till have some. And because experimental samples are less, it need be approved by farther experiment or studies. 2. The antibodies compositive type of + + + have a remarkable effect on the treatment outcome. Therefore, it is shown that the infection of CT can influence the treatment outcome of the infertile patients of IVF-ET treatment when the organisms damage...