The Experimental Study Of Nerve Fragments Join Laminin For Promoting Peripheral Nerve Defects Repair

ObjectiveTo observe the nerve fragments join laminin induce peripheral nerve defcts repair role for the clinical encounter cases of peripheral nerve defects to find a way of convenient and feasible restoration.Materials And Methods1.PLGA composite tube PreparationTake a certain amount of approximately 100,000 molecular weight polymer soluble in chloroform, the concentration 10% of the solution, until the polymer completely dissolved, add a certain amount of sodium chloride by grinding screening, and fully stirred after mixing and pouring in the mold, standing 24-48 hours after pouring, Demoulding after standing 24-48 hours, and then in a vacuum drying oven drying for 24 hours, the final will be material under deionized water immersion stirring from time to time, each 4-6 hours for a water, for 48 hours, removed do seep through water, vacuum drying for 24 hours. The tube was 85% porosity, pore size of about 200-300 microns, diameter 1.8mm, outer diameter 3-4mm. The tube were produced by the author and DaiGang Biology Technology Ltd.2.Animal experimentWistar rats 30,male, body weight 200g around,randomly divided into 3 groups, each with 10.Anesthetize by ketamine in abdominal,reval left leg sciatic nerve in sterile conditions,cut apart the nerve in the piriformis margin of about 3mm,which is the sciatic nerve stem about 6mm,retract as their natural.Group A : put autologous smashed nerve fragments into the PLGA tube ,8/0 non-invasive suture closure of the outer membrane and PLGA wall so that making the two ends of the PLGA tube from 10 mm .Group B: Laminin(10ug/ml)was added into the PLGA tube after conduit never defect within never fragments.Group C:we suture the cutted never stem with 8/0 non-invasive .suture the wounds and breed one group by one cage .Analysis of results1.general observationAfter two weeks ,All the rat's experimental side toes feet close together and can not be a walk-shaped and swollen with varying degrees, non-ulcer formation. Rats two weeks after wound healing and suture off. After eight weeks, neural transplantation and nerve fragments + LN group can be a toe-side test, the gastrocnemius muscle atrophy with varying degrees, show toe can lead to reflection; nerve experiment fragments yet to be a toe-side, the gastrocnemius muscle atrophy obviously, reflection can not lead to toe.12 weeks after nerve graft and nerve fragments + LN group outreach toe there, show a more sensitive reflection toe, with varying degrees of gastrocnemius muscle recovery; neural fragments group toe can walk, no toe outreach phenomenon, atrophy of gastrocnemius muscle more pronounced, did not show toe leads to reflection. PLGA can be seen eight weeks after catheter adhesion with surrounding tissue loose, easily separated from the surface of a small amount of capillary formation, cut open the duct and neural neural debris debris + LN group PLGA nerve regeneration tubes are passed,Autologous nerve graft nerve graft group can be seen with mild adhesions surrounding tissue, near and far end there was no anastomotic expansion near normal nerve appearance. Catheter after 12 weeks of PLGA degradation has been completed, the formation integrity epineurium, cut open the outer membrane can be seen fragments Group nerve than the neural fragments + LN group slightly smaller diameter nerve regeneration, nerve regeneration debris nerve + LN group with more rules, no obvious fibrous tissue hyperplasia. Autologous nerve graft group can be seen close to normal appearance,no expansion of anastomotic distance,anastomotic and non-adhesion of surrounding tissue, no overflow of nerve fibers, The activity of transplantation of nerve nearly be normal.2.resultBy detecting EMG gastrocnemius, triceps surae muscle wet weight, regenerative nerve biopsy study Microstructure and image analysis, the results was applied with SPSS13.0 for one-way ANOVA and t test, P <0.05 for statistical significance, analysis shows: 8 and 12 weeks, the nerve fragments and LN group of neurons to restore function is superior to neural fragment group P <0.05, autologous nerve graft group was significantly better than the nerve fragments groups P <0.05, with the nerve fragment + LN group was not significant statistically significance of P> 0.05.Conclusions1.Experiments confirmed that PLGA tube has a good biocompatibility, biodegradation rates of appropriate,is a good peripheral nerve tissue engineering materials.2.Nerve fragments can be used as nerve conduit bridging the filler to promote peripheral nerve repair the defect.3.Experiments Confirmed that nervefragments + LN groups to restore the effect of nerve close to or be able to reach the level of autologous nerve graft for the treatment of peripheral nerve defect with a new method.