Mechanism Study On Lung A549 Antipersonnel Effect By PVP Technology With Polymtthy Methacrylate ¹²⁵I Seed

Objective:Primary study of antipersonnel effect to iodine-125 combination Polymtthy methacrylate to lung cancer vertebral column transfer A549 cells,from fundamental research evidence-based Clinical Results.Methods:The irradiator consist of nine¹²⁵I seeds,each with 0.8Ci of apparent activity.Eight seeds were eaually spaced around the circumference of 3-cm diameter circle and ninth seed was confined to the center.Pour the 3 ml Polymtthy methacrylate into polystyrene foam well-propotioned then LungA549 cell line were placed 5mmabove the seed plane and delivered.LungA549 cell line is plant in the Petri dish(diameter 35mm),when the cells adherent,while irradiated by¹²⁵I seeds.The cells were divided into 4 groups(n=6) randomly:control group(A group),¹²⁵I seed group(B group),Polymtthy methacrylate group(C group),¹²⁵I seed group and polymtthy methacrylate group(D group),the control group cells(no irradiation)were cultured 24 hours,48 hours and 72 hours respectively.¹²⁵I seed group:the cells were irradiated by 24hours,48 hours and 72 hours respectively.Polymtthy methacrylate group:the cells were irradiated energy emission by 24 hours,48 hours and 72 hours respectively.¹²⁵I seed group and polymtthy methacrylate group: Irradiated and energy emission by 24 hours,48 hours and 72 hours respectively,and then we collected the specimens and frozen them respectively.We detect the cell apoptosis and necrosis rate with flow cytometry and observe cells form changed by clairvoyant electron microscopy.Results:Lung A549 cells were exposed by continuous low-dose irradiation of ¹²⁵I seeds.The rate of apoptosis was determined by flow cytometry.The result showed that the apoptosis rates of the control group cells were(1.94±0.06)%(n=6,time=24h) and the apoptosis rates of the control group cells were(2.07±0.19)%(n=6,time=48h) and the control group cells were(2.25±0.19)%(n=6,time=72h).The apoptosis rates of the ¹²⁵I seed group cells were(10.91±1.22)%(n=6,time=24h),and apoptosis rates of the ¹²⁵I seed group cells were(14.72±0.97)%(n=6,time=48h),the apoptosis rates of the ¹²⁵I seed group cells were(18.38±1.35)%(n=6,time=72h).The apoptosis rates of the Polymtthy methacrylate group cells were(3.46±0.10)%(n=6,time=24h),the apoptosis rates of the Polymtthy methacrylate group cells were(3.80±0.12)% (n=6,time=48h),apoptosis rates of the Polymtthy methacrylate group cells were (4.03±0.11)%(n=6,time=72h).Apoptosis rates of the ¹²⁵I seed group and Polymtthy methacrylate group cells were(15.99±1.91)%(n=6,time=24h),the apoptosis rates of the ¹²⁵I seed group and Polymtthy methacrylate group cells were (19.03±2.20)%(n=6,time=48h),theapoptosis rates of the ¹²⁵I seed group and Polymtthy methacrylate group cells were(22.34±1.79)%(n=6,time=72h).The result of clairvoyant electron microscopy showed that the control groupcells have no apoptosis,apoptosis can be seen in ¹²⁵I seed group,Polymtthy methacrylate group,¹²⁵I seed group and polymtthy methacrylate group significantly.Through detection showed that ¹²⁵I seed group and polymtthy methacrylate group cells apoptosis rates increased obviously.Conclusion:1.¹²⁵I seeds can promote apoptoisis in lung A549,and cells apoptosis and necrosis rates are higher with extended radiation time.2.The heating effect of polymtthy methacrylate can lead to cell apoptosis3.¹²⁵I seeds with Polymtthy methacrylate lung A549 antipersonnel effect can promote apoptosis and necrosis rates with extended radiation time.