| Objeetive:Stretch the brain by brain retractor, use microdialysis technique to monitor local cerebral tissue, Dynamic collection of extracellular fluid (ECF) in the biochemical substances. Research different brain retraction pressure and different brain retraction manner affect the biochemical metabolism of regional cerebral tissue by brain retraction. Microdialysis monitoring of brain stretch in local metabolic changes in brain biochemistry potential applications.Methods:Take 10 adult dogs,10 dogs were divided into groups and their own brain traction control. Intravenous infusion of craniotomy, cut the dura mater, brain plate under different traction force (20mmHg,30mmHg,40mmHg), different traction mode (continuous traction, intermittent traction) on the temporal lobe for traction. Application of microdialysis dynamic collection of local dog brain extracellular fluid stretch (ECF) of dialysis fluid. Observed glucose ([Glu]d), lactate ([Lac]d), glycerol concentration ([Gly]d) and glutamic acid ([Gluta]d) changes, and calculate the lactate /pyruvate ratio (L/P).Results:①respectively 20mmHg,30mmHg,40mmHg of traction force on the brain tissue to stretch, pull to remove 1 hour before dialysis, after pulling every 0.5 hours to collect a dialysis fluid, were collected 5 hours. Biochemical analysis of the dialysis liquid, pressure in different brain traction caused varying degrees of brain extracellular fluid in the [Glu]d, [Lac]d, [Gly]d, [Gluta]d change. After brain traction [Glu]d decreased to varying degrees, between the traction group were compared with the control group and pull a significant difference compared with before (P＜0.05). [Lac] d in the brain increased after stretch, the stretch between groups were compared with the control group and pull a significant difference compared with before (P＜0.05). [Gly]d in the brain slowly increased after the stretch, the stretch between groups were compared with the control group and pull a significant difference compared with before (P＜0.05). [Gluta]d in the stretch after the earlier changes in extracellular fluid, the traction group had different degrees, between the traction group were compared with the control group and pull a significant difference compared with before (P＜0.05). L/P ratio in range, and each group compared with the control group and pull significantly different than before (P＜0.05)②different brain stretch mode (continuous traction and intermittent traction) on the brain organizations pull, continuous traction ECF in [Glu]d, [Lac]d, [Gly]d, [Gluta]d, L/P ratio of change is more obvious, continuous traction and intermittent traction compared, and with the control group and pull a significant difference compared with before (P＜0.05).Conclusions:1. Traction forces generated in different brain ECF in Glu, Lac, Gly, Gluta content and L/P ratio changes in different degrees, Glu content decreased, Lac, Gly, Gluta, and L/P ratio is increased in varying degrees, Brain pulling force increases, the change is obvious. This change in brain tissue and brain plate after stretch of regional cerebral ischemia related.2. With different traction mode (continuous traction and intermittent traction) on the brain tissue to stretch, continue to pull in brain tissue than the brain of intermittent stretch produced a more severe brain damage. Brain is better than intermittent traction is due to persistent brain stretch every few minutes, brain tissue has a short reperfusion, cerebral ischemia is relatively light.3. Microdialysis technique is a minimally invasive, safe, new monitoring tools, able to stretch the local brain biochemical changes of substance continuous, dynamic metabolic monitoring.4. Microdialysis technique can be used as monitor in the neurosurgery brain damage produced by stretching a valuable tool.
|
PDF Full Text Request