Regional Cerebral Metabolism Changes After Brain Injury: A Microdialysis Study

Objective:Through the dog's brain injury model,the use of microdialysis in brain injury and sampling different time slots of the regional analysis.Blood for metabolism of certain substances in the dynamic content changes.Such as glucose, lactic acid,glycerol and so on.Understanding of brain injury from these substances, such as changes of metabolism and its clinical significance,and to explore the microdialysis technique in the local biochemical brain injury monitoring the use of substances.Method:From 6 adult healthy dogs,anesthesia,surgical fenestration in the exposed dura mater.Approach to the production of free-fall impact epidural mild and severe brain injury model,the application of microdialysis technique to collect dynamic constant partial brain damage ECF,collected every 30min interval 1,injured before the three specimens were collected.6h after injury,collected 12 specimens.At the same time to contralateral normal brain tissue as a reference to the collection of dialysate for analysis Goryeong,traumatic brain injury in order to observe the state of glucose(Glu),lactate(Lac),pyruvate(Dyr),glycerol(Gly) content such as material and its dynamic changesResults:Through the microdialysis method for detection of extracellular concentrations of Glu and Lac was observed after brain injury Glu decreased with decreased significantly compared to the contralateral reference,2-3 hours after injury, reached a peak,followed by a gradual pick-up in Lac.Content after the injury and the changes in Glu contrary.However,slower changes.ECF brain Glu and Lac in the content of the degree of change and injury,the more damage the more obvious changes in weight,longer duration can be used as to determine the extent of injury and brain edema an important basis;also prompted lactate/glucose ratio(L/G) more obvious changes and better reflects the brain tissue redox status and the degree of ischemia.Glycerol did not change significantly,the degree of light-related injury.Conclusion:1.Microdialysis technique is a minimally invasive,safe,new monitoring tools,to partial brain damage and biochemical changes in the material content of the continuing monitoring of the metabolism.2 after brain injury decreased ECF glucose,lactic acid content increased,both were contrary to the dynamic changes that may be due to brain damage brain tissue glycolysis led to a significant enhancement.3.Monitoring after brain injury in ECF glucose and lactic acid changes can determine the condition and prognosis as an important biochemical indicators.4 Glu and Lac more obvious changes in the ratio can be used as a more sensitive indicator of the potential.