Correlation Of Blood Lipids And Inflammation Cytokines In Patients With Coronary Heart Disease And Periodontal Disease

Objective:1. Established China Han's survey information base, the clinical database and biological sample database of three types of population following. (1) associated with coronary heart disease patients with periodontitis. (2) systemically healthy periodontitis patients, including chronic periodontitis and aggressive periodontitis. (3) healthy controls.2. A preliminary study of coronary atherosclerotic heart disease (CHD) and periodontitis relations in the serum.Methods:1. Periodontal examinationIn accordance with diagnostic criteria, select periodontitis with coronary heart disease patients, with coronary heart disease patients, with moderate and severe periodontitis patients and healthy control group. Record periodontal index: include calculus index-simplified, bleeding on probing, probing depth and attachment level.2. Specimens collectedFasting 10ml,3500r/min centrifugal 15min (centrifugal radius of 8 cm), separated serum and collected in sterile test tube, sealed shading,-70℃low temperature refrigerator stand-by.3. Lipids, blood glucose measurement Enzymatic determination of total cholesterol(TC) and triglyceride(TG); the direct determination of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Use automatic blood chemistry analyzer (Hitachi 7060) analysis serum biochemical indicators; use automatic blood chemistry analyzer (Olmpus Au640 Japan) analysis serum glucose (blood glucose, Glu) levels.4. Laboratory testingUsing ELISA method, Power Wave 340 PCR Analyzer (United States, BIO-TEK Inc.) in the OD values obtained at 450nm, using a CurveExpert software draw standard curves, than according to the sample OD value in the software calculates the corresponding serum IL-6, TNF-a and IL-1(3 levels. The level of CRP uses immune turbidimetric method (Toshiba 7060 automatic biochemical analyzer) to test.5. Statistical analysisSPSS 13.0 analyzed using statistical software, measurement data use (mean±standard deviation). Calculus index in each group was use rank sum test; AL, PD into a group design was used to compare t-test; BOP positive rate use chi-square test; blood glucose, serum IL-6, CRP, TNF-a and IL-1βlevel use single-factor analysis of variance and least significant difference multiple comparisons. Detection level of bilateral a= 0.05.Results:1. Calculus index (CI-s) comparison:The rank and testing obtained shows, (C+P) and C group compare with C and H group respectively, there are significant differences(p< 0.01); There are no statistical significance between (C+P) and P groups (p> 0.05).2. PD, AL and BOP positive rates comparison:(C+P) group of PD and AL were significantly greater than P, C and H group (p< 0.05). The BOP positive of (C+P) group was significantly higher than C group, H group (p< 0.01) and P group (p< 0.05).3. Lipid levels, IL-6, IL-1β, CRP, TNF-a levels comparison:The HDL-C level of (C+P) and C group are lower than P and H group(p< 0.05); Compared of LDL-C, TC, TG levels among these four groups, there are no statistically significant difference (p> 0.05); The IL-6, IL-1β, CRP, TNF-a level of (C+P) group was significantly higher than P, C and H group.4. The blood glucose level of all patient groups were higher than that of control group, the blood glucose level of (C+P) group is the highest, was statistically significant difference(p< 0.05); The blood glucose level of C group and (C+P) group was significantly higher than H group (p< 0.01).Conclusion:1. The inflammatory mediators (IL-1β, IL-6, TNF-a, etc.) of periodontal infection played a role in these two diseases, and they can also affect each other.2. Dyslipidemia in coronary heart disease associated with chronic periodontitis, their periodontal pockets of bacteria and their LPS-induced toxic ingredients can be further reduced HDL-C concentration in the blood, and reduce the amount of HDL cholesterol metabolism, which would led to coronary heart disease and increase the possibility.3. Periodontal infections will affect the endocrine metabolism and thus affect the blood glucose control and increase the risk of complications associated with diabetes.