Effect Of Matrine On STAT1,3,CTGF,PDGF Expression In Fetal Glomerular Mesangial Cells Under LPS Condition

Objective:Primary culture embryos glomemlar mesangial cell(MC) of human. To determine,3,CTGF and PDGF in glomemlar mesangial cell induced by lipopolysaccharide(LPS),and to explore the protective mechanisms of Ma.METHODS:Primary cultured human embryos glomemlar mesangial cell was identified.Ma were added to cultured glomerular mesangial cells which were exposed to LPS for 12,24 and 48 hours respectively.Real time polymerase chain reaction(Real time PCR) were used to determine STAT1,3,CTFG,PDGF mRNA.The protein expression of STAT1,3 and p-STAT1,3 were observed by Western blot.RESULTS:Compared with control group,MC proliferation of the LPS group (10μg/ml) was increased significantly,while the Ma(320μg/ml)group was decreased at 12,24,48 hours(P＜0.01).The expression of STAT1,3,CTGF,PDGF mRNA and the levels of p-STAT1,p-STAT3 protein were significantly increased in MC under LPS medium at 12,24,48 hours,while in Ma group were obviously lower than those of the LPS control group especially at 24,48 hours.CONCLUSION:The protective mechanisms of Matrine is considered to down-regulation STAT1,3,CTGF and PDGF.