The Study Of The Protection Of Methylprednisolone Sodium Succinate On The Heart Function Of The Brain Death Donor

Objective::To study the protection of Methylprednisolone Sodium Succinate on the heart function of the brain dead pig donor by increasing intracranial pressure intermittently and slowly to build pig brain-death model,while giving Methylprednisolone Sodium Succinate to the brain-dead pig.Methods:To randomized 14 healthy Xishuangbanna Miniature pigs into hormonegroups and control groups. The groups were gived general anesthesia and then carride on tracheotomy and tracheal cannula, mechanical assistance, internal carotid venous cannula, craniotomy and placed Foley balloon catheter into intracranial, by increasing intracranial pressure intermittently and slowly to build pig brain-death model for 10 hours. Ringer solution and Dextran-20 gived to the control groups, Methylprednisolone Sodium Succinate additional intravenous infusioned for the hormone groups.The datas of the heart rate,MAP,CVP,LVEF and total volume of urine(TVU) were recorded, and made myocardium into pathological section simultaneously.Results:1.The model of the pig brain-death has been sucessfully established. The success ratio was 85.7% and the living survival ratio was 100%. The death of the models were overdose of anesthesia(befor model,1) and took place ventricular fibrillation frequently (between model,1).2.The changes of haemodynamics:by increasing intracranial pressure intermittently and slowly to build pig brain-death model, in this progress the blood pressure rised rapidly,heart rate increased fastly in the early stage and then appeared peak blood pressure, the thing followed was the pressure backed to normal level even though increased intracranial pressure,this time, the model was sucessfully established. Anaphase left ventricular ejection fraction(LVEF) reduced, to keep the basic blood pressure we had to use vasoactive agents.The LVEF and mean arterial pressure(MAP) significantly increased in the hormone groups compared with the control groups(P<0.05). The research showed that Methylprednisolone Sodium Succinate can improve the function of the brain-dead pigs significantly.3.The changes of biochemical indicators:the change of neuroendocrine after the brain dead led to the injury of body metabolism, including thyroid function. In our study after the brain dead 1hour, the concentration of T3,T4,TSH gradually decreased alongwith the progress.While the fall degree significantly slow in the hormone groups compared with the control groups(P<0.05) and before the brain dead(P<0.05).4.The pathological section of myocardium(HE):the control groups,cardiac muscle cell hypertrophy nonuniformly, elongated,cytoplasm and muscle fibril stained shoally, inflammatory cells infiltration and haemorrhagia local visual; the hormone groups, cardiac muscle cell hypertrophy, elongated,cytoplasm and muscle fibril stained shoally, unseen inflammatory cells infiltration or haemorrhagia.Conclusion:1.Success of the animal model is the parameter clsuse for the experimental conditions. Only through training and increasing skilled experience, we can improve the survival rate of animal models, to ensure the smooth progress of the experiment in the next step.2.The haemodynamics is one of the methods that evaluate the heart function at present, the improvement of the haemodynamics is the symbol of the improvement of cardiac function.After set up the model 10hours, the blood pressure,cardiac fraction of the hormone groups were better than the control groups, and the urine volume were less than the control groups, keep internal environment steady state easily.So we think that Methylprednisolone Sodium Succinate plays an important role in the the protection of the heart function of the brain dead pig donor.