Proteomics Analysis Of Tongue Squamous Cell Carcinoma 8113 Cells In Hyperthermia-induced Death

[Objective] Analyse protein expression profile of tongue squamous cell carcinoma cells 8113 in different time,Discuss the mechanism of heat kill tongue squamous cells in proteo-mics level.[Methods]Routineculture human tongue squamous Tca8113 cells into six groups.One group is blank controller,others groups are disposed for Celsius heat treatment method of water with 40min 43 degrees Celsius,and then put cells in couveuse for 2 hours,6 hours,8 hours,12 hours and 24 hours with 37 degrees Celsius.After collecting cells we extracte total proteins. We get protein expression profile with 2-DIGE. Analyze data collected from suppliers, MALDI-TOF-MS.At last,we get proteins knowledge in SwissProt and NCBInr.[Results] Two dimensional gel electrophoresis patterns of plasma proteins with high resolu-tion and reproducibility were successfully obtained using DIGE.One hundred and five signifi-cantly differentially expressed protein spots were screened with DeCyder 6.5 software. Fifty-three proteins were successfully identified via peptide mass print using MALDI-TOF/MALDI-TOF/TOF.Swissprot data base:(1)Basic metabolism related enzymes:CoA:3-ketoacid-coenzyme A Succinyl-transferase 1,mitochondrial;Fructose-bisphosp hate aldolase A;Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase,mitochondrial;Glyceraldehyde-3-phosphate dehydrogenase,Pyruvate kinase isozymes M1/M2,Phosphoglycerate kinase 1,26S proteasome non-ATPase regulatory subunit 14, ATP synthase subunit d, mitochondrial,Pyridoxine-5'-phosphate oxidase, Hetero-geneous nuclear ribonucleoprotein H,Galactokinase,Argininosuccinate synthase;(2)Cytoske-leton class:Tubulin beta chain,Vimentin,Keratin,typeⅡcytoskeletal 8, Keratin, typeⅡcytos-keletal 7 and Keratin, typeⅠcytoskeletal 10,Actin, cytoplasmic 2,F-actin-capping protein su-bunit beta;(3)Stress related proteins:Protein disulfide-isomerase A3;Glutathione S-transferase P,Alpha-enolase;(4)Signal transduction molecules:Cofilin-1;(5)molecular chaperone:HSP60, HSPβ1,HSP70,HSP71,Prohibitin,T-complex protein 1 subunit zeta;(6)Transcription and trans-lation related proteins:Far upstream element-binding protein 1,Eukaryotic translation initia-tion factor 5A-1,Splicing factor U2AF 65 kDa subunit;(7)Phosphorylation related proteins: Vimentin,Cofilin-1,Lamin-A/C,Serum albumin,Proliferation-associated protein 2G4,Mitotic check-point protein BUB3;(8)Apoptosis related proteins:Cofilin-1,Pyruvate kinase isozymes M1/M2,Eukaryotic translation initiation factor 5A-1,HSP60,HSP70;(9)Others:Clathrin light chain A,Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase,mitochondrial, Inorganic pyrophos-phatase,D-3-phosphoglycerate dehydrogenase,Scavenger mRNA-decapping enzyme Dcp. NCBInr data base:(1)Basic metabolism related enzymes:peroxiredoxin-4,Phosphoglycerate mutase 1,3-hydroxyacyl-CoA dehydrogenase type-2 isoforml,flavin reductase, peroxiredoxin 6, ALDOA;(2)Cytoskeleton class:Keratin, type II cytoskeletal 8;(3)Stress related proteins: stathmin 1 oncoprotein18,stathmin 1,BolA-like protein 2,(4)Signal transduction molecules: Chain A,Crystal Structure Of The Ufc1,Ufin1 Conjugating Enzyme 1,Chain A,Human Trans-lation Initiation Factor Eif1a,Chain A,Crystal Structure Of Human Eukaryotic translation Ini-tiation Factor Eif5a;(5)Others:protein 4.1 isoform 3,hCG15971,isoform CRA_b.[Conclusion] There are many regulatory factors when heat inducting Tca8113 cells apop-tosis. There are difrrent regulatory factors in different times.