| Background and objective:With the increasing aging population and work to accelerate the increase in competitive pressure, cerebral infarction has become a serious threat to the current health of the major diseases in the elderly. With an increasingly higher level of medical and health conditions improve, cerebral infarction significantly reduced mortality, but cerebral dysfunction after severe neurological sequelae (eg, hemiplegia, aphasia, mental retardation, difficulty swallowing, depression, etc.) to patients, families and the community a heavy burden. To improve the quality of life of patients and explore effective treatment measures for studying hot spots. Studies have shown that electrical stimulation can promote limb motor function in rats with cerebral infarction and learning and memory capacity to restore. Clinical study found that during the development of the nervous system, bone morphogenetic protein (BMP) and its receptors for the development of the process of cell differentiation, survival, reproduction and neurotransmitter phenotype, etc. has important regulatory functions in the mature the central nervous system, also has its own receptor expression. This study in rats after cerebral infarction in rats electrical stimulation of the hemiplegic limb motor function recovery and in vivo effects of BMP secretion, which may provide a new theoretical basis for the application of electrical stimulation in stroke treatment and rehabilitation. randomly divided into electric stimulation, control, sham operation and normal groups (the number of each group is 48,48,48,8).Acute cerebral infarction model was mimicked using middle cerebral artery occlusion techniques. The control group received natural recovery; Electrical stimulation group received a daily natural recovery and a hemiplegic limbs of electrical stimulation, Every 30 minutes one day. At 6h,12h,24h,3d,7d,21d time point, eight rats in each group to take to carry out walking the balance beam test, rats were observed functional recovery of hemiplegic limbs, and then decapitated rat brain tissue taken fixed, slice, add BMP antibodies, determination of positive expression of the average gray matter values, the final color by immunohistochemical positivity.Results:Treatment of the first 7d,21d, the electrical stimulation to restore limb function compared with control group. The normal group and sham operation group BMP2,6,7 expression was not statistically significant. The control group and the electrical stimulation expression of BMP-2 are first down, electrical stimulation of rat BMP-2 down time is earlier than in the control group at 21d to resume to normal levels, The control group and the electrical stimulation BMP-6 were raised, and maintain a high level, electrical stimulation control group; BMP-7 level of performance for the first downward and then upward, to the 21d returned to normal levels, electrical stimulation peaks higher BMP-7 expression.Conclusion:1,After middle cerebral artery occlusion of the rats'left brain, the right limbs have been completely paralyzed; the motor function of paralyzed limbs begin to improve among each group from the 7d after operation, with indicator that motor function can be improved spontaneously after acute cerebral infarction.2,Electrical stimulation therapy can promote the paralyzed limbs of rats with cerebral infarction recovery of motor function, can induce large cerebral infarction in rat brain BMP-2,6,7 factor secretion. 3,we speculate the mechanism of ameliorating motor function may involve the up-regulation or down-regulation of BMP-2,6,7expression upon electric stimulation in acute cerebral infarction. |
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