Study Of Oxidative Stress In Chronic Hepatitis B Patients With Elevated Serum Total Bilirubin

Objective This experiment mainly investigate changes of antioxidation ability and oxidative stress in chronic hepatitis B(CHB) patients with elevated serum total bilirubin(TBIL), through measuring Malondialdehyde(MDA), Xanthine Oxidase(XOD),Vitamin C(VC) and Vitamin E(VE) in the serum. This experiment can guide clinical works for the future and may help to improve the effect of therapy in the clinic.Methods 75 CHB patients with elevated serum TBIL were enrolled in the present study. They had been admitted to our hospital and accorded with diagnosis criterion publicized at 2000, include 33 females and 42 males whose ages from 18 to 59. These selected patients have not other diseases. A,B,C,D and E group were defined as 1～2,2～3,3～5,5～10 and more than 10 times of upper limit of normal (ULN) serum TBIL in the patients. The control group contained 11 healthy donors include 5 females and 6 males whose ages from 18 to 50 and the carrier group contained 16 Hepatitis B surface antigen (HBsAg) carriers include 7 females and 9 males whose ages from 21 to 51.These selected persons have not any disease. Serum MDA, XOD, Vitamin C and Vitamin E were determined by chemic colorimetry.Results 1.There were 21 cases whose serum TBIL 1～2times of ULN,19 cases whose serum TBIL 2～3times of ULN,12 cases whose serum TBIL 3～5times of ULN,11 cases whose serum TBIL 5～10times of ULN and 12 cases whose serum TBIL more than 10 times of ULN in the patients.2. The concentration of XOD was significantly higher in the carrier group than in the control (P< 0.05), while MDA was not significantly higher, VC and VE were not significantly lower (P>0.05). 3. The concentrations of MDA and XOD were significantly higher in each group of patients than in the control (P< 0.05), while Vc and VE were significantly lower (P < 0.05).4. The concentration of MDA was significantly higher in each group of patients than in the carrier group (P< 0.05). The concentration of XOD was significantly higher in group B, C, D, E of patients than in the carrier group (P< 0.05). The concentration of Vc was significantly lower in group B, C, D, E of patients than in the carrier group (P< 0.01). The concentration of VE was significantly lower in group D, E of patients than in the carrier group (P< 0.05).5. The concentration of MDA was significantly different among all the groups in the patients except the comparison between group A and group B. The concentration of XOD was significantly different between group A, B, C and group D, E (P<0.05). The concentration of VC was significantly different between group A and group D, E and between group B, C, D and group E (P<0.05). The concentration of VE was significantly different between group A, B and group E (P<0.05).6. The concentrations of MDA and XOD were significantly positively correlated with TBIL (r= 0.670, P< 0.01; r=0.737, P< 0.01, respectively) in the patients, while VC and VE were significantly negatively correlated with TBIL (r=-0.463, P< 0.01; r=-0.247, P< 0.05, respectively).Conclusions 1.There was a disturbance between oxidative stress and anti-oxidative ability in CHB patients with elevated serum TBIL. Oxidative stress became more serious along with the increasing of serum TBIL.2. In HBsAg carriers, oxidative stress level was low.3.The results suggest antioxidant treatment for CHB patients with elevated serum TBIL may help to improve the effect of therapy.