The Influence Of PTEN Decent On Biological Ability Of Breast Cancer Cell

The breast cancer is a common malignant tumor in female, but the detailed mechanism is yet unclearly. Phosphatase and tensinhology deleted from chromosome 10 (PTEN), also named mutated in multiple advanced cancers (MMAC1) or TGF regulated and epithelial cell-enriched phosphatase 1 (TEP1), is the first anti-oncogene with phosphatase activity and is cloned by three different research groups such as Steck. PTEN is located at 10q23. In many tumors, it is often loss of heterrozygosity. Many researches showed that the breast cancer is closely related to the loss or desent of Pten protein。Normal expression of PTEN can inhibit the invasion, metastasis and growth of tumor cell. But if it is unnormal, the protein will expressed decreased and even loss. The loss of PTEN gene connected with cell signal transduction, tumor pathogenesis, invasion and metastasis, malignant transformation, unlimited growth and clinical outcome. The present researches found: PTEN plays an important role in breast cancer. Its aberrance will cause breast overgrowth and occurred tumor earlier, while the wide PTEN overexpression inhibit the growth of breast. Some researches reported that wide PTEN accelerated the cell apoptosis to inhibit the breast cancer cell growth and caused its death by decreased PI3K level and induced G2 phase.Now the researches on PTEN gene and its expression, signal transduction and the role in many tumors bocomes a hot point. Further studies on its role and mechanism in breast cancer ant the interaction with other genes had important clinical meanings in diagnose, treatment and prognosis estimate at gene level.Based on aboved theory, to research the expression of PTEN and its influence on biological ability in breast cancer cell in vivo, we designed this experiment. We cultured the M231 and H293T cell in DMEM/F12 and high DMEM respectively. Then PTEN-shRNA plasmid was transtected into M231 breast cancer cells to knock down the expression of PTEN. The changes of PTEN expression, proliferation, adhesion and metastasis of PTEN knocked down cell were tested by western-blot, colony formation, adhesion and invasion assay.Results:PTEN-shRNA was successfully transfected into M231 cells and PTEN expression was efficiently inhibited. Compared to the control group, the experiment clones showed a greater capibility of colony formation, migration and invasion. But the transfected cells were more difficulty in adhesion than the scrambled one. Thers is significantly difference between the two groups.(P<0.01)Conclusion:PTEN gene can enhance the adhesion, but restrict the proliferation, migration of breast cancer cells in some degree, so that inhibit the development of the breast cancer. PTEN loss can be a prognostic factors for the patients with breast cancer.