The Research Of Protein Expression Of HCN4 Gene In Atrial Fibrillation Patients Of Rheumatic Heart Disease

Objective: Through the analysis of HCN4 gene expression in rheumatic heart disease patients with mitral valve stenosis and in control group ,we aim to explore the possible adjustment mechanism of atrial fibrillation in mitral valve stenosis of rheumatic heart disease and to lay the theoretical foundation of atrial fibrillation.Methods: Through Western Blot quantitation to study HCN4 gene expression at two group rheumatic heart disease patients with mitral valve stenosis, one group with atrial fibrillation as experimental group and the other without atrial fibrillation as control group. We used the ratio of HCN4 gene expression and theβ-actin mRNA expression for evaluating the mRNA expression level of HCN4 gene.Results: By Western Blot analysis of atrial fibrillation patients with mitral valve stenosis of rheumatic heart disease and control group, the ratio of HCN4 gene expression andβ-actin mRNA expression values ( x±s) were 0.29±0.03 and 0.13±0.03 (p<0.01) respectively, which indicated that the HCN4 gene expression significantly rise in atrial fibrillation patients with mitral valve stenosis of rheumatic heart disease.Conclusion: 1.HCN4 gene expression in atrial fibrillation patients with mitral valve stenosis of rheumatic heart disease increases, which may affect the hyperpolarization-activated cation current (If), resulting in atrial fibrillation.2. Controling atrial HCN4 gene expression and regulating hyperpolarization-activated cation current (If) probably prevent atrial fibrillation appearence and stop atrial fibrillation maintenance.