Effect Of Mangiferin On The Invasive Ability Of Leukemia HL-60 Cells

Objective To study the effect of mangiferin on the adhesion and invasive ability of human acute myeloid leukemia HL-60 cells and its possible mechanism, further to study the melocular mechanism for treating leukemia.Methods The cell adhesion assay and the Minicell chamber assay were performed to determine the effect with different concentration of mangiferin for 24 and 48 hours on the adhesion and invasive ability of HL-60 cells. The expression of FAK,CXCR4 and MMP-2 mRNA was detected by semi-quantitative RT-PCR.Results①The results of cell adhesion experiment showed that the cell adhesion ability inhibited in HL-60 cells in dose-time dependent manner after treated by 20-250μmol/L mangiferin for 24,48 hours.②Mangiferin efficiently suppressed the results of Chamber experiment showed that the number of cells under micro-membrane decreased in HL-60 cells in dose-time dependent manner after treated by 20-250μmol/ L mangiferin for 24,48 hours.③Mangiferin efficiently suppressed the expression of FAK,CXCR4 and MMP-2 mRNA in HL-60 cells, the expression of FAK mRNA in dose-time dependent manner. The expression of CXCR4 and MMP-2 mRNA not statistical significance after treament with mangiferin for 24 hours exposure of HL-60 cells from 20μmol/L. It efficiently suppressed from 40μmol/L and the proportion of which with 160μmol/L and 200μmol/L mangiferin was significantly lower than that occurring in untreated cells. It's not statistical significance that expression of CXCR4 and MMP-2 genes treated with mangiferin for 48 hours and high concentration but it's statistical significance compare with that occurring in untreated cells. (P<0.05).Conclusion Mangiferin can inhibits obviously the invasive ability of leukemia HL-60 cells and its mechanism may be related to inhibit obviously the capability of adhesion and reduce the expression of FAK CXCR4 and MMP-2 mRNA.