Expression And Significance Of Apoptosis Genes, Cell Adhesion Mclecules, Proteases Associated With Tumor Invasion, And Angiogenic Factors In Gastric Carcinoma Lauren's Classification

ObjectivesTo investigate the expression and significance of apoptosis genes (Bcl-2, Bax) , cell adhesion molecules (E-cad, β -cat) , proteases associated with tumor invasion (MMP-9) , and angiogenic factors ( VEGF) in gastric carcinoma Lauren's classification, and the relationship between incomplete large intestinal metaplasia, HP infection, and Lauren's classification of gastric carcinoma. This study was aimed to explore the role of carcinoma-related genes in the progression of the two subtypes of gastric carcinoma, and relationship of biological behaviors of gastric carcinoma and gene-expression, and distinctive genetic process through which the two subtypes of gastric carcinoma developed. MethodsImmunohistochemistry streptavidin-peroxidase method was performed in gastric carcinoma and adjacent normal gastric mucosa. The positive expression rates of these genes coding proteins were investigated and compared with clinicopathological parameters, at the same time, HID-AB and Warthin-starry histochemistry method were performed to test the relationship between incomplete large intestinal metaplasia, HP infection, and Lauren's classification of gastric carcinoma.Results1 The expression of Bcl-2 coding protein was lower in the normal gastric mucosa than in gastric carcinoma, while the greater expression of Bax, on the contrary, was seen in the normal gastric mucosa, and the difference was significant (P<0.01). Both the expression of Bcl-2 and Bax were significantly correlated with the Lauren's classification of gastric carcinoma. Bcl-2, specifically, had a greater expression in diffuse-type gastric carcinoma, compared to Bax, a lower expression in diffuse-type gastric carcinoma. Besides, there was correlation between the positive expression of Bcl-2 and the depth of invasion which, however, was not observed in Bax. Both of them were not significantly associated with the metastasis of lymph node.2 The normal expression of E-cad and P -cat was seen in all of the adjacent normal gastric mucosa, while the abnormal expression of both was found in gastric carcinoma to varied extent. A significant correlation was observed between the abnormal expression of E-cad, P -cat, and the Lauren's classification, The abnormal expression rate was higher in the diffuse-type than in the intestinal-type of gastric carcinoma. The abnormal expression of ￡ -cat was closely correlated with the invasion depth of gastric and lymph node metastasis, regarding E-cad, however, such correlation was not found.3 The positive expression of MMP-9 was nearly not seen in adjacentnormal gastric mucosa, while increasing expression was seen in gastric carcinoma region, the difference was statistically significant (P<0.05). The expression of MMP-9 was closely related to the depth of invasion, and metastasis of lymph node (p<0.05), while there was no significant association with Lauren's classification.4 The low expression of VEGF was seen in adjacent normal gastric mucosa, while increasing expression was seen in gastric carcinoma region, the difference was statistically significant (p<0.05). The expression of VEGF was closely related to the depth of invasion, and metastasis of lymph nodes (p<0.05), while there was no significant association with Lauren's classification.5 The positive expression rates of the incomplete large intestinal metaplasia and infection of HP were not significant associated with both subtypes of gastric carcinoma according to Lauren's classification.Conclusions1 Incomplete large intestinal metaplasia and infection with HP were seenin both subtypes of gastric carcinoma, which indicated a insignificant difference in the etiology and environmental factors.2 In the growth and progression of gastric carcinoma, due to the distinctive mutations of the cell of diffuse-type gastric carcinoma gained different additional genes (e.g. Bcl-2 and Bax, E-cad and P...