Study On The Effects Of NXKKFY On Both The CNP, 6-keto-PGF1α, TXB₂ Of Rats Suffering From Myocardiallschemia And Their Cardiac Muscle Tissue

Coronary heart disease is also known as ischemic heart disease. The three main western medicine treatment methods for coronary heart disease include medication, interventional therapy and surgery, all of which have their own shortcomings. In recent years, the relationship between endothelial dysfunction and coronary heart disease has been considered to be of great importance. Endothelial dysfunction exists in the pathogenesis of coronary heart disease and it exists even before the attack of coronary heart disease. Coronary heart disease is caused by a combination of many factors, and one of the important reasons is the imbalance of the synthesis and release of vaso-active substances - which means the increase of the synthesis and release of the constriction vaso-active substances in contrast to the reduce of the synthesis and release of the dilation vaso-active substances. C-type natriuretic peptide (CNP), thromboxane B2 (TXB2), 6-keto-prostaglandin F1α(6-K-PGF1αa) are vaso-active substances that have been discovered in succession in recent years. A thorough understanding of the relationship between endothelial dysfunction and coronary heart disease can reduce the incidence of acute coronary heart disease and reduce the fatality rate of its patients.Coronary heart disease is the equivalent of Chest Pain in traditional Chinese medicine, whose symptom is asthenia in origin and asthenia in superficiality and whose pathogenesis is heart vessel blockage stasis. When it attacks, the main symptom is asthenia in superficiality and protrusion of blood stasis, whereas during remission the main symptom is deficiency of both yin and yang and ischemia and hypoxia of the heart, spleen and kidney, among which the most common symptom is myocardial hypoxia. In recent years, with the further study on the theoretical research on qi and blood in traditional Chinese medicine, the method of supplementing qi and activating blood circulation is increasingly attached to great importance and highly valued, and many physicians'in-depth study has confirmed that many compound preparation of traditional Chinese medicine can have a good effect on the prevention and the treatment of the coronary heart disease and can delay the its onset process.Bu Yang Huan Wu Decoction (BYHWD) is a good prescription for the treatment of hypoxia and blood stasis. Based on BYHWD, the experts of Traditional Chinese Medicine of the First Clinic Hospital of Jilin University have developed NXKKFY by removing peony root, peach kernel, and carthamus tinctorius linne, using Dang Gui in place of Dang Gui Wei, and adding caulis polygoni multiflori, lignum millettiae, fleece-flower root, the root of bidentate achyranthes, Ji Sheng, lycopodium clavatum, notopterygium root, and the seed of cowherb, etc. It can not only retain the efficacy of supplementing qi and activating blood circulation but also can clear and activate the channels and collaterals as well as invigorate heart and kidney. NXKKFY derives from the pathogenesis of"hypoxia and blood stasis", and based on the theory of"Qi is the commander of blood; blood is the mother of qi; qi can activate the blood; blood can be a carrier of qi; activating qi can thus activate blood; and stagnation of qi can result in blood stasis", it develops from the rules of treatment of"supplementing qi and activating blood circulation as well as activating meridians to stop pain". It can be mainly used to prevent and treat the cases of Chest Pain of the hypoxia and blood stasis type, with the efficacy of supplementing qi, activating blood circulation and dissolving stasis. This prescription can increase the amount of qi and make the blood unimpeded, and thus the blood stasis can be eliminated, new blood can be regenerated, and heart meridian can be unblocked. In this way the prescription can have the efficacy of supplementing qi and activating blood circulation as well as dissolving stasis and clearing and activating the channels and collaterals.Through the building of animal models, this experiment investigates the sheltering effect of NXKKFY on rats suffering from myocardial ischemia injury and the influence of NXKKFY on CNP,TXB2,6-K-PGF1αin the serum of such rats, so as to provide a theoretical basis for clinical medication.The methods adopted were assorting the rats into six groups randomly, which were NXKKFY large doses, middle doses and small doses groups, positive control group (control group of Composite Salvia Dropping Pill), negative control group (model group of myocardial ischemia) and normal control group. Then NXKKFY large doses, middle doses and small doses groups took concentrate solution of NXKKFY through intragastric administration, with the respective quantity as much as the clinic recommendation quantity of 400%, 200% and 100%. Positive control group were given Composite Salvia Dropping Pill, and normal control group and negative control group were given the same quantity of normal saline water through intragastric administration. The rats of each group were given the above-mentioned medicines through intragastric administration once a day for 20 days continuously. On the 21st day, one hour after giving the respective medicines through intragastric administration, the rats of the first five groups were given an lumbar injection of isoproterenol and the rats of the normal control group were given an lumbar injection of the same quantity of saline water. 24 hours later, the lumbar injection of isoproterenol or saline water was given again in exactly the same way. Two hours after the building of the models, all rats were killed in order to observe and analyze the changes in the content of CNP, TXB2 and 6-K-PGF1αin their serum, and to observe the histopathological changes from the hematoxylin and eosin stain of pathological sections.The results are as follows: 1. The change of the content of CNP: Compared with the normal control group, the content of CNP increases significantly in the negative control group. This proves that CNP is of importance in myocardial ischemia injury. Compared with negative control group, the following four groups - NXKKFY large doses, middle doses and small doses groups and positive control group - all can reduce the CNP significantly. Among these four groups, the NXKKFY large doses group has better capability to reduce the CNP than other NXKKFY doses groups and positive control group, as can be proved through significant differences in statistics. As indicated above, NXKKFY can significantly reduce the content of CNP in rats suffering from myocardial ischemia and thus can have sheltering effect on the myocardial ischemia of rats. 2. The change of the content of 6-K-PGF1α: Compared with the normal control group, the content of 6-K-PGF1αreduces significantly in the negative control group. This proves that 6-K-PGF1αis of importance in myocardial ischemia injury. Compared with negative control group, the following four groups - NXKKFY large doses, middle doses and small doses groups and positive control group - all can increase the content of 6-K-PGF1αsignificantly. Among these four groups, the NXKKFY large doses group has better capability to increase the 6-K-PGF1αthan other NXKKFY doses groups and positive control group, as can be proved through significant differences in statistics. The results indicate that NXKKFY can significantly increase the content of 6-K-PGF1αin rats suffering from myocardial ischemia and thus can have sheltering effect on the myocardial ischemia of rats. 3. The change of the content of TXB2: Compared with normal control group, the content of TXB2 increases significantly in negative control group. This proves that TXB2 is of importance in myocardial ischemia injury. Compared with negative control group, the following four groups - NXKKFY large doses, middle doses and small doses groups and positive control group - all can significantly reduce the TXB2. Among these four groups, the NXKKFY large doses group has better capability to reduce the TXB2 than other NXKKFY doses groups and positive control group, as can be proved through significant differences in statistics. The result implies that NXKKFY can significantly reduce the content of TXB2 in rats suffering from myocardial ischemia and thus can have sheltering effect on the myocardial ischemia of rats. 4. Pathological changes: Put the pathological sections of the myocardium tissue of each group of rats under light microscope (HE×100) and the following changes can be observed:Normal control group: Cardiac muscle fibers are arranged in neat rows; cell shape is essentially normal; cell nucleus and cytoplasm are clear; striations are clear; and there are no significant histological changes. Negative control group: Cardiac muscle fibers are swollen and broken in many places; striations vanish partially; tissue space is widened; there are denaturation and losses in cell nucleus; and there is interstitial edema. Positive control group: Cardiac muscle fibers are less neat; a small amount of karyopyknosis can be observed; striations are less clear; there is a little interstitial edema; tissue space is widened slightly. NXKKFY large doses group: Cardiac muscle fibers are arranged comparatively neat; there is no denaturation in cell nucleus; striations are clear; there is no obvious interstitial edema; and the shape of interstitial substances is close to normal. NXKKFY middle doses group: Cardiac muscle fibers are arranged basically completely; cell nucleus is relatively clear; striations are clear; there is no obvious interstitial edema. NXKKFY small doses group: Cardiac muscle fibers are less neat; there is no denaturation in cell nucleus; striations are less clear; there is no obvious interstitial edema; tissue space is widened a little bit.These changes imply that, compared with negative control group, all the NXKKFY large, middle and small doses groups and positive control group can reduce the injury of the cardiac muscle tissue, and among all these groups the NXKKFY large doses group has better capability in this aspect than other NXKKFY doses groups and positive control group. This indicates that NXKKFY can have a sheltering effect on rats suffering from myocardial ischemia.The results of the experiments show that NXKKFY can significantly increase the 6-K-PGF1αcontent and decrease the CNP and TXB2 content in the serum of rats suffering from myocardial ischemia. This indicates that NXKKFY can effectively protect the ischemic myocardium and can significantly prevent coronary heart disease.