The Investigation Of Tissue Engineering Corneal Stoma Based On Bacterial Cellulose Scaffold

Corneal blindness is the eye disease which cased blind secondary to cataract.Currently,the most effective way of therapy is keratoplasty with homogeneity variant cornea.The most corneal blindness is still suffer from blind because of the short of fresh corneal stuff and the rejection after corneal transplantation .Artificial tissue engineering coenea is hopeful to cure blind by substitute the vital cornea. The scaffold material is still the hot point and restrictive factors of tissue engineering cornea.Bacterial cellulose (BC) is a kind of natural biological nanodimensional material.BC owe many special property as bioscaffold such as high crystallinity,high chemical purity,high machanical strength,high gas permeability , water penetation,reasonable biocompatibility and well control during manufacture.It has been successfully used in food industry and paper industry.For the past few years,BC was used in medical material and tissue engineering more and more widely.But it have not been searched in the use of tissue engineering cornea internal and abroad.In order to investigate if BC can be used in tissue engineering corneal stroma ,the study use upgrade BC membrane which can make corneal stromal cells grow better on ground of early research.Then do animal experiment.First of all,observe the physical composition of BC scaffold through scanning electron microscope .Second,transplant BC mambrane without corneal stromal cells into rabbit corneal stromal pocket.Analysis biocompatibility,transparency and degradation of BC membrane by slit lamp microscopy OCT, Cs-4 in vivo and pathology , immunohistochemisty,SEM ex vivo in different time after grafting. ThenChoose the left eye of rabbit corneal stroma.Seperate and culture the corneal stromal cells.Mark the cells with BrdU.Plant the third generational cells on BC mambrane. Transplant the compound membrane to the homobody.Analysis biocompatibility and posibility of BC membrane as scaffold of tissue engineering coenea stroma by slit lamp microscopy OCT, Cs-4 in vivo and pathology , immunohistochemisty,SEM ex vivo in different time after grafting. Conclusion:1.It was observed that the aperture of improved BC mambrane is bigger by SEM..So it's good for corneal stromal cells to grow inside the mambrane.2.BC need more improvement already.Harmful substance during manufacture should be removed as far as possible.3.Separate,culture and transfer the rabbit corneal stromal cells ex vivo. Plant the cells which grown better to the BC mambrane.Observe the stromal cells by mark and immunohistochemisty.The cells grown well.It proved that BC is safe and cell compatible as natural biological nanodimensional material.4. After transplanting the BC mambrane with and without corneal stromal cells into rabbit corneal stromal pocket.Although neovessels was observed at the beginning it decreased and retracted gradually.And the cornea is almost transparent.BC did't degradated yet observed by OCT, pathology and SEM.But confused with normal corneal stroma gradually.It's important to research.It means well biocompatibility and transparency of BC.It's a kind of potential tissue enginering corneal scaffold material.In conclusion,the study used the improved natural biological nanodimensional material BC as scaffold of tissue engneering corneal stroma.We found that the biocompatibility of BC is well..It's a kind of perspective tissue enginering corneal scaffold material. The study offered new thread for tissue engineering and expanded research area of BC.