The Relationship Between EGFR Gene Amplification And The Response Of Gefitinib In Patients With Advanced Non-small-cell Lung Cancer

Background:EGFR tyrosine kinase inhibitors Iressa(gefitinib) block the activity of EGFR by competing ATP biding the ATP-bingding pocket on EGFR,EGFR as their targets,and they are hotspots in the field of treatment of advanced NSCLC.Researches had reported that the Amplification rate of EGFR gene was much higher in Iressa responsers than in non- responders.The tumor relief rate was much higher in patients with EGFR amplification than in those without EGFR amplification.The overall survival of NSCLC patients with EGFR amplification had a longer trend than that of patients without amplification,but had no stastical significance.Now the most common method to detect EGFR gene amplification was direct sequencing,expensive and time consuming,which didn't fit clinical screening.Aim:The study was to evaluate that EGFR increases in NSCLC rate and to the EGFR tyrosine activating enzyme inhibitor curative effect relevance.Method:This research has collected the later period NSCLC patient's pathology organization wax block which 63 examples accept Iressa the treatment. Hybridizes the (FISH) technology with the fluorescence home position to examine the EGFR gene.Results:63 example NSCLC specimen Chinese Communist Party examines in 33 example specimens to have the EGFR gene to increase, increasing rate is 52.4%. EGFR gene increasing rate in the female, the adenocarcinoma, does not smoke in the patient high. The EGFR gene increases the patient to accept the Iressa treatment effectiveness to be higher than not increases the patient (54.5%vs16.7%, p=0.014). Has patient's disease inverse amplification factor which the EGFR gene increases to be higher than not increases the patient (86.4%vs54.1%, p=0.011), has statistics significance.Conclusion:Has later period NSCLC which the EGFR gene increases to accept the Iressa treatment effectiveness and disease inverse amplification factor is higher than the non-EGFR gene mutation, and has statistics significance.