The Study Of Lentiviral Transfection Of Stromal Stem Cells Derived From Rabbit Bone Marrow

Objective:To research the effective labeling technique for tracing MSCs.Method: Take MSCs as objective for study,aspriates bone marrow blood from iliac crest and separated by adherent cell cytopheresis.MSCs were cultured in vitro and collected to experiment.293FT packaging cells were transfected by the constructed plasmids PWPXLd/PAX2/PMD2.G to acquire lentivirus with EGFP gene.Transfected the MSCs with lentivirus and use fluoromicroscope to observe the results.Collected MSCs ,measure the transfected efficiency by flow cytometry.Results: we separated and cultured rabbit marrow stromal stem cells in vitro and transfected it by lentivirus with EGFP gene.Conclusion: Acquired rabbit marrow stromal stem cells and transfected them with lentivirus vector of modified gene.Make a foundation for gene engineering and tissue engineering.