Constitution Of Animal Model For Aortic Valved Patch Homograft And The Morphological Change Of The Patch After Implantation

ObjectiveTo investigate the influence of aortic and pulmonary homografts on host and the effect of immunological rejection on the homografts. Rat model of cryopreserved aortic patch homograft heterotopic transplantation was established to investigate the effect of cryopreservationg on the antigenticity of the aortic patch homografts, to study the effect of immunosuppressive treatment on the aortic and pulmonary homograft transplantation.Materials and MethodsIn this study,Liquid nitrogen cryopreservation method was adopted in the long-term storage of the aortic patch homografts. The animal models of aortic patch homograft transplantation were established by heterotopic insertion of aortic f SD rats into abdominal aortae of Wistar rats. Implantation of valved homograft,objects were classified randomly into intervention group,immunological compared group and empty compared group. The biological activities of IL-2 and the ratio of inner layer thickness to media layer thickness of the transplanted vessels were measured by 3days,7days,14days,and 21 days after implantation.The biological activities of IL-2 in blood samples of the implanted rats were measured by means of ELISA.Resultsintervention group and immunological compared group were all thickend in the inner layer of the transplanted vessels by 7days(P<0.05).But in intervention group,the changes was greater than in the immunological compared group(P<0.05). There were apparent increase of biological activities of IL-2 in both intervention group and immunological compared group(P<0.05). Conclusion1. There was significant rejection after implantation of valved patch homografts.2.The treatment of CsA can suppressive the expression of IL-2 on immunological rejection.