| ObjectiveBy this study we hope to analysis the clinicopathology of childhood lymphoma according with the tumours of haematopoietic and lymphoid tissues of World Health Organization Classification of Tumours(2008).We hope to analysis the clinicopathologic features of childhood lymphoma and relationship with EBV infection and the genetic change of c-myc gene in childhood lymphoma.Methods75 cases of childhood lymphoma were selected from the files of Pathology Department,Chinese Medical University Shengjing Hospital in period of 1996 to 2009.Pathologic diagnosis was according to the WHO classification for tumors of hematopoietic and lymphoid tissue(2008).Immunophenotype analysis was performed by SP method.Antibodies selected were included CD20, CD79a, CD3, CD45RO, Ki67,CD21,TDT,CD10,BCL6,MUM1,ALK,CD30,EMA and CD15.ISH were used for detection of EBER in all the cases. FISH were used for detection of the segregation of c-myc gene in BL tumor and DLBCL tumor.ResultsClinicopathologic features:there were 57 cases of males and 18 cases of females in 75 cases of childhood lymphoma, the ration of sex was 3.17:1, the average age was 8.74 years old.There were 32 cases (42.7%,32/75) in lymph node,43 cases (57.3%,43/75) in extra-node,including 22 cases (29.3%,22/75) in gastrointestinal tract, 3 cases (4.0%,3/75) in mediastinum,2 cases (2.7%,2/75) in thymus gland,2 cases (2.7%,2/75) in testis,2 cases (2.7%,2/75) in tonsil,2 cases (2.7%,2/75) in bone marrow,2 cases (2.7%,2/75) in brain,2 cases (2.7%,2/75) in soft tissues,2 cases (2.7%,2/75) in striated muscle,1 case (1.3%,1/75) in basivertebral,1 case (1.3%,1/75) in liver and spleen,l case (1.3%,1/75) in skin,l case (1.3%,1/75) in pelvic wall. The most common subtypes were Hodgkin lymphomas (17.3%,13/75),Lymphoblastic lymphoma (21.3%,16/75),Anaplastic large cell lymphoma (16.0%,12/75),Burkitt lymphoma and Diffuse Large B-cell Lymphoma (44.0%,33/75), Small lymphocytic lymphoma (1.3%,1/75). The detection of EBV:There were 14 cases were positive (18.7%,14/75),including 11 cases of Hodgkin lymphomas (84.6%,11/13),3 cases of Burkitt lymphoma and Diffuse Large B-cell Lymphoma (9.1%,3/33).The genetic change of c-myc gene was executived by FISH in cases of BL tumor and DLBCL tumor.18 cases of BL tumor exists the c-myc gene segregation in 28 cases of Burkitt lymphoma and Diffuse large B-cell lymphoma.ConclusionsThe cases of male was more than those of female,the average age was 8.74 years old.There were more cases in lymph node and gastrointestinal tract.The most common subtypes were Burkitt lymphoma, Lymphoblastic lymphoma, Hodgkin lymphomas, Anaplastic large cell lymphoma and Diffuse Large B-cell Lymphoma. The positive rate on EBV of Hodgkin lymphomas is as high as in the literature,showed childhood Hodgkin lymphoma was significantly associated with EBV infection.The positive rate on EBV is lower in BL and DLBCL,showed in childhood BL and DLBCL EBV were only the concomitant phenomenon or only a predisposing factor.
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