Effect Of Magnetic Iron Oxide Nanoparticle-mediated IASPP Antisense Gene On Proliferation And Apoptosis In Human Pancreatic Cancer Cell Line SW1990

ObjectiveTo determine the effect of magnetic iron oxide nanoparticle-mediated iASPP (inhibitory member of apoptosis stimulating protein of p53) ASODN (antisense oligo deoxynucleotides) on proliferation and apoptosis in human pancreatic cancer cell line SW1990.Materials and methodsDextran coated magnetic iron oxide nanoparticles modified with polylysine (pll-DCIONP) was prepared as an oligodeoxynucleotide carrier to transfect iASPP ASODN into human pancreatic cancer cell line SW1990.There were five groups in this study as followed:group ASODN-pll-DCIONP, group SODN-pll-DCIONP, group ASODN, group pll-DCIONP and group SW1990.The MTT assay was used to detect the proliferation of SW1990 cells and apoptosis was detected by flow cytometry(FCM).Results1.Growth of SW1990 cells was inhibited by ASODN-pll-DCIONP in a concentration dependent manner from 0.5μmol/L to 2.0μmol/L,and compared with the other study groups (group ASODN-pll-DCIONP, group SODN-pll-DCIONP, group ASODN, group pll-DCIONP and group SW1990) the differences were statistically significant.While the concentration of ASODN-pll-DCIONP was 1.0μmol/L,growth inhibition rates of SW1990 cells were recorded respectively at 24h,48h and 72h after transfection,and the rates gradually decreased.2.FLC showed that ASODN-pll-DCIONP increased the apoptotic rates of SW1990 cells in a concentration dependent manner from 0.5μmol/L to 2.0μmol/L, and compared with the other study groups the differences were statistically significant. While the concentration of ASODN-pll-DCIONP was 1.0μmol/L, apoptotic rates of SW1990 cells were recorded respectively at 24h,48h and 72h after transfection,and the rates gradually decreased.Conclusion1.Growth of SW1990 cells was inhibited by ASODN-pll-DCIONP and apoptosis was increased in a concentration dependent manner.2.Effect of ASODN was enhanced when mediated by pll-DCIONP.3.The longer after transfection,the lower the growth inhibition rates and the apoptosis of SW1990 cells.