Studies On The Pharmacy Of Flos Puerariae

The study of this paper was mainly on the basis of dried flowers of Pueraria lobata(Willd.) Ohwi. Via the literature research of Flos Puerariae, chemical constituents study, herbal medicine quality standard study, extraction and purification process of total flavonoids in pilot testing study parts were investigated to control the quality of this medicinal materials effectively, find out the material basis of main drug action.In chemical constituent studies, five compounds were isolated from Flos Puerariae.by determining physical constants,showing the color of the chemical reaction and using TLC,MS,1H-NMR,13C-NMR to analysis, tectorigenin,genistein,daucosterol,tectoridin and tectorigenin-7-O-xylosylglucosid were identified. Tectorigenin,tectoridin and tectorigenin-7-O-xylosylglucosid were the highest concentration in Flos Puerariae, which can be used as reference constituents in quality control of Flos Puerariae and its preparation. The more systematic study of chemical constituents of Flos Puerariae is necessary in future.In herbal medicine quality standard study, the study established the criteria of quality control of Flos Puerariae, for it was not consummated in the former criterion which had only morphological and histological identification. The quality standard was incomplete which no TLC and content determination presented, so Flos Puerariae of drug was studied on preliminary pharmacy.10 different areas of samples of Flos Puerariae were collected to establish the criteria of quality control of Flos Puerariae.TLC qualitative testing,determination of total flavonoids by ultra-voilet spectrophotometry and quantitative analysis by RP-HPLC were conducted to 10 samples from different habitats, using tectorigenin,tectoridin and tectorigenin-7-0-xylosylglucosid as reference substance. These methods developed in this experiment can be used for the quality control of Flos Puerariae.Extraction and purification process of total flavonoids in pilot testing study, Studies were carried according to the technological parameters of extraction and purification process of total flavonoids in laboratory experiment. Pilot testing study of extraction process was followed as:13.33BV 60% ethanol distilling three times,1.5h per time, merging filtrate of three times, at low temperature-high speed centrifugal (4℃,5000r/min, 10min) discarding water insoluble. Pilot testing study of purification process was followed as:XDA-1 macroeticular resins, herbal medical materials to dried macroeticular resins being 3:4, herbal medical materials concentration being 0.08g/mL, eluted by 30BV 50% ethanol after 25BV water.50% ethanol part was collected and dried under vacuum(48h) resulting in the total flavonoids of Flos Puerariae content reaching 51.80%. This study provide centain theoretical guide line for manufacture.