Study On Correlation Between Vitamin D Receptor Genetic Polymorphism And 25-hydroxyvitamin D In Rickets

Objective To study the correlation between vitamin D receptor genetic polymorphism Fokâ… and vitamin D deficiency rickets in children of 1-3 year old, and to explore the significance of hereditary factors in the development of vitamin D deficiency rickets.Methods This was a case control study. The subjects were composed of two groups, the patient group consisted of 62 children with vitamin D deficiency rickets, the control group was composed of 60 healthy children. The level of 25-hydroxyvitamin D3 in both groups was analyzed by Enzyme-Linked Immunosorbnent Assay (ELISA). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genetic analysis method was used. A restriction fragment length polymorphism in the vitamin D receptor genetic polymorphism Fokâ… was tested in both groups. The frequencies of the vitamin D receptor genotype and allele were compared between the two groups.Results There was a significant difference in the serum 25-hydroxyvitamin D3 between two groups (t=-6.696, P<0.05). The FF genotype and F allele were more common in patient group than in control group, respectively, There was a significant difference in the frequency distribution of vitamin D receptor genotype and allelic genes between two groups (x2= 10.221, P<0.05;x2= 7.511, P<0.05).Conclusion There is a correlation between vitamin D receptor genetic polymorphism Fokâ… and vitamin D deficiency rickets. This study suggested the possibility that vitamin D receptor genetic polymorphism might be important in determining susceptibility to development of vitamin D deficiency rickets.