Effect Of Ketamine Postconditioning And Ketamine Postconditioning Combined With Ischemic Postconditioning On Hepatic Ischemia-reperfusion Injury In Rats

Objective To investigate the effect of ketamine postconditioning and it combined with ischemic postconditioning on hepatic ischemia-reperfusion injury in rats.Methods Twenty-four male SD rats weighing 200-230g were divided into 4 groups:group 1, sham-operated group(sham group, n=6); group 2, ischemia-reperfusion injury group(IR group, n=6); group 3 ketamine postconditioning group(ket group, n=6); group 4 ketamine postconditioning combined with ischemic postconditioning group(ket+Ipo group, n=6). According to Nauta RJ's method to establish a model of ischemia-reperfusion injury. After 1 h ischemia, group 3 and group 4 were administrated ketamine(10mg/kg), but group 4 were administrated ischemic postconditioning at the same time. Blood samples were obtained after reperfusion 4 hours for determination of serum ALT, AST; livers were removed for determination of express of Bcl-2, Bax and MDA, SOD; also for electronic microscopic examination in each group.Results Group 3 and 4 compared with group 2, ALT, AST, MDA and the express of Bax were significantly lower(P<0.05), SOD and the express of Bcl-2 were higher(P<0.05). The results of electronic microscopic examination also demonstrated the injury of group 3 and 4 were lighter than group 2. There weren't different between group 3 and 4, including ALT, AST, SOD, MDA and the result of electronic microscopic examination.Conclusion Ketamine postconditioning could reduce the ischemia-reperfusion injury, but that it was combined with the ischemic postconditioning didn't enhance the protective effect.