Comparison With Different Frequency Of Cycles Of Ischemic Postconditioning That Affects Liver Reperfusion Injury In A Rat Model Of Remnant Liver After Partial Hepatectomy

Objective：To explore the effect of treatment with repeated cycles ofischemic postconditioning in hepatic ischemia reperfusion injury ofremnant liver after partial hepatectomy.Methods：Sixty-four healthy SD rats of clean grade， weighing220g~280g， were randomly assigned to six groups:70%partialhepatectomy group（PH），70%partial hepatectomy combined withischemia reperfusion group（PHIR）， two cycles of60s~60s ischemicpostconditioning group（IPO1）， four cycles group（IPO2）， six cyclesgroup（IPO3）， eight cycles group（IPO4）. The inferior vena blood andthe remnant liver tissues were harvested6h after reperfusion to bedetected with activities of AST and ALT of serum and index AI ofhepatocytes.Results： Compared with those in PH group serum AST and ALTactivities and hepatocyte AI in PHIR groups were all increased（P<0.05）.Compared with PHIR group serum AST and ALT activitiesand hepatocyte AI in PHIR groups were all decreased （P<0.05）.Conclusion： Ischemic postconditioning can attenuate ischemiareperfusion and its mechanism is related to inhibiting oxidative stress as well as reducing production of free radicals and infiltration ofinflammatory cells.