Expression Of NF-κB,ICAM-1 After Cerebral Ischemic Reperfusion And The Effects Of Artificial Synthetic E-selectin In Rats

0bjective: To study the mechanisms of nuclear factor-кB and intercellular cell adhesion molecule-1 in rat brain suffered with ischemia-reperfusion injury and to further explore the protective mechanism of artificial synthetic E-selectin in cerebral ischemia-reperfusion injury model of rats.Methods: The model of middle cerebral artery occlusion (MCAO)was established by thread ligation method.Healthy male Sprague-Dawley rats were randomly divided into 3 groups: sham operation group, operation group, and artificial synthetic E-selectin treated group, with 36 in each group. Samples were divided into 6 groups (2h, 6h, 12h, 24h, 48h and 72h after reperfusion). The contents of nuclear factor-кB and intercellular cell adhesion molecule-1 expression in rat brain were measured by immunohistochemical methods, and intercellular cell adhesion molecule-1 mRNA was detected by reverse transcription-PCR. The experimental data were analyzed by the Statistical Analysis System version 8.0 software, and were tested at the level of hypothesis testα= 0.05 andα= 0.01 respectively.Results: 1. The sham operation group: The expression of NF-кB,ICAM-1 and ICAM-1 mRNA were very small at different time points, and keep invariable with time going. 2. Operation group: It was found that NF-кB appeared at 2h post-operation, increased at 6 and 12h, reached peak at 24h,and then decreased gradually at 48 and 72h. The expression of NF-кB in operation group was more than sham operation group (P <0.01) at each time point. ICAM-1 was detected at 2h post-operation, reached peak at 48h, and then decreased gradually at 72h. The expression of ICAM-1 in operation group was more than sham operation group ( P<0.01) at each time point. ICAM-1mRNA were also detected at 2h post-operation, reached peak at 48h, and then decreased gradually at 72h. The expression of ICAM-1mRNA in operation group was more than sham operation group ( P<0.01) 3. At each time point The varied trends of NF-кB in artificial synthetic E-selectin treated group was the same as operation group, but decreased much obvious at each time point (P<0.05). It showed the similar results with ICAM-1 mRNA and protein expression .Conclusions: 1. NF-кB induces ICAM-1 expression at transcriptional level in rats suffered with cerebral ischemia-reperfusion injury. 2. Artificial synthetic E-selectin can protect the ischemic brain tissue by decrease the expression of ICAM-1. 3. The protection mechanism of artificial synthetic E-selectin for cerebral ischemia-reperfusion injury is probably that it inhibits the synthesis of ICAM-1 mRNA which is induced by NF-κB at the transcriptional level, resulting in decreasing the expression of ICAM-1.