| Objective: To observed the change of coagulation system of the acute radiation sickness, extremely severe bone marrow form acute radiation sickness with 8.0 Gy 60Coγray irradiated Wistar rats, moderate bone marrow form with 3.0 Gy 60Coγray irradiated rhesus monkeys and severe bone marrow form with 2.3 Gy neutron irradiated beagle were used. The therapeutic effects on the hemorrhage of recombinant human granulocyte colony-stimulating factor (rhG-CSF) to the three animal models were evaluated.Methods: Complete blood counts and platelet aggravate were monitored after radiation. In the coagulation test, activated partial thromboplastin time (APTT) shows the activity of the intrinsic pathway of coagulation, prothrombin time (PT) reflects the extrinsic pathway of coagulation, thromboplastin time (TT) indicates the end stage of the clotting cascade and fibrinogen concentration (FIB) was observed. In thrombelastogram (TEG) R (reaction time; time to clot initiation), K (rate of clot development), MA (maximum amplitude) and LY60 (Fibrinolysis after 60 min) was observed.Results: APTT decreased to 81%,TT decreased to 86%, R decreased to 89%compared to the value before radiation in the early stage post radiation(3d) of 8.0 Gy 60Coγray irradiated Wistar rats. APTT decreased to 99%,TT decreased to 91%, R decreased to 65%compared to the value before radiation in the early stage post radiation(3d) of 2.3 Gy neutron irradiated beagle. Consider the results together in the extremely severe bone marrow form and severe bone marrow form acute radiation sickness thrombosis increased and blood coagulation has been strengthened. In the very period of acute radiation sickness (about 11d after irradiation in rats,14d to 21d after irradiation in monkeys and 14d after irradiation in Beagle),WBC,RBC and platelet count decreased, platelet aggregation decreased. R value prolonged, thrombosis rate (1/K) decreased, MA reduced and LY60 decreased wneh TEG performed in the very period of acute radiation sickness. APTT was shortened to 66% compared to the value before radiation in 8.0 Gy 60Coγray irradiated Wistar rats; PT was decreased to 85.53% compared to the value before radiation in 2.3 Gy neutron irradiated beagle; APTT was shortened to 90% compared to the value before radiation 3.0 Gy 60Coγray irradiated rhesus monkeys. Fibrinogen concentration increased both in 8.0 Gy 60Coγray irradiated Wistar rats and 2.3 Gy neutron irradiated beagle after radiation.R and K both increased and MA deceased in high radiation dose rate group than lower radiation dose rate group with 8.0 Gy 60Coγray irradiated Wistar rats. rhG-CSF promoted WBC, RBC and PLT recovery, speed up the rate of thrombosis, increased MA and Fibrinogen concentration in the 8.0 Gy 60Coγray irradiated Wistar rats.rhG-CSF strengthened platelet aggregation function, decreased R, PT and TT in 3.0 Gy 60Coγray irradiated rhesus monkeys.rhG-CSF improved the survival rate, decreased R and K time, increased fibrinolysis rate, decreased Fibrinogen concentration of neutron irradiation Beagle. Conclusions: Coagulation strengthened in the early stage after irradiation. Platelet count declined progressively and platelet aggregation decreased after irradiation. In the very period of acute radiation sickness thrombosis decreased but there is a certain degree of increased in the coagulation test. The damage of coagulation system in the high radiation dose rate is serious than the lower radiation dose rate group. RhG-CSF not only promote blood cell recovery but also accelerate thrombosis.
|
PDF Full Text Request