Spermatogenesis Effect Of Testicular Torsion On The Contralateral Testis In Immature Rats

Objective:To observe the spermatogenesis effects of unilateral testicular torsion on the contralateral testis in immature rats. and compare the results of different treatments.Methods:Thirty-two immature male Wista rats were randomly divided into 4 groups(n=8)and Testicular torsion models were established in 4-week-old rats. Group 1 was sham operation group. group 2 to 4 were experiment groups. Unilateral 720°testicular torsion was performed on the rats for two hours in group 2 or six hours in group 3 and 4 before detorsion. Resection of ipsilateral testis to the rats in group 4 at the time of detorsion. Six weeks after detorsion. the contralateral testes were removed and weighted. The effects on spermatogenesis of the contralateral testes were evaluated with the weight, pat hological, apoptosis of germ cell and DNA flow cytometric.Results:1 The contralateral testes displayed atrophy and sclerosis in group 2 to 4 of the 10-week-old rats.2 Interstitial edema and ultrastructure changes were observed, It can be seen a little germ cell apoptosis and FCM histograms showed lower peak of apoptosis in group 2 to 4.3 The right testis weight was significantly greater in group 2 to 4 than in group 1(P<0.01), No significant differences were noted in the right testicular Germ cell apoptosis index, Proportion of haploid cells and Mean seminiferous tubule diameter among the four groups(P>0.05).Conclusion:1 Unilateral experimental testicular torsion in immature rats could cause compensatory hyperplasia of spermatogenesis in the contralateral testes.2 The opposite side to avoid injure by early management after testicular torsion.