Influences Of Smoking And Smoke Quitting On Serum Leptin, Adiponectin, IL-6 And CRP In Rats

Objective:The objective of this paper is to investigate the role of inflammatory factors like serum leptin, adiponectin, interleukin-6 (IL-6) and C-reactive protein (CRP) in the systemic inflammatory response of smoking-induced COPD.Methods:32 Male Wistar rats are randomly divided into four groups:group of rats smoking heavily, group of rats smoking fewer, group of rats smoke abatement and a control group, with 8 rats for each group. Rat smoking device was in accordance with Xusanlin smoking device.Except the rats of the control group, all the other rats smoke 2 times one day,6 days one week, for 20 weeks.The first group and the third group smoke 10 cigarettes each time, while the second group 5. The third group of rats quit smoking for 10 weeks after smoking 20 weeks. Serum leptin, adiponectin, IL-6 and CRP levels are measured by ABC-enzyme-linked immunosorbent assay(ELISA).Results:1. Leptin:Compared to the control group[(128.00±13.25)pg/ml], serum leptin levels of the rats in the other three groups [(56.23±8.64) pg/ml; (64.13±10.36) pg/ml; (60.36±7.42) pg/ml respectively] decrease significantly (all P<0.05); compared to the first group, the difference is not significant in both the second group and the third group (P> 0.05 respectively)2. Adiponectin:Compared to the control group[(0.369±0.032) ng/ml], serum adiponectin levels of the rats in the other three groups[(0.322±0.045) ng/ml; (0.327±0.024) ng/ml; (0.333±0.059) ng/ml; respectively] decrease significantly (all P<0.05); compared to the first group, the difference is not significant in both the second group and the third group (P> 0.05 respectively)3. IL-6:Compared to the control group, serum IL-6 levels of the rats in the other three groups[(39.67±3.13) pg/ml; (36.58±2.43) pg/ml; (34.27±7.01) pg/ml respectively] increase significantly (all P<0.05); serum IL-6 levels of the rats in the second group and the third group are lower than those of the first group (P<0.05 respectively)4. CRP:Compared to the control group, serum CRP levels of the rats in the other three groups [(809.50±141.66) ng/ml; (628.91±128.83) ng/ml; (642.60±180.55) ng/ml respectively] increase significantly (all P<0.05); serum CRP levels of the rats in both second group and the third group are lower than those of the first group (P<0.05 respectively)5. Correlation Analysis:Adiponectin levels are inversely related with IL-6 and CRP (r=-0.392, P＜0.01; r=-0.631, P＜0.05 respectively)Conclusions Smoking reduces the levels of serum leptin and adiponectin but increases the levels of serum IL-6 and CRP of rats, further more, serum IL-6 and CRP levels are positively related with smoking status. After smoke quitting, levels of serum leptin and adiponectin are still lower than those fo the control group, while IL-6 and CRP levels decrease, but both of them are higher than those of the control group. What's more, adiponectin level is inversely related with IL-6 and CRP. These results suggest that inflammatory factors such as leptin, adiponectin be involved in the systemic inflammatory response of smoking-induced COPD.