Expression Of MiR-21 And Its Association Of PDCD4 In The Laryngeal Squamous Carcinoma

【Objective】:To undersand the expression of miR-21 and PDCD4 and discuss their relationships in the laryngeal squamous carcinoma.【Method】:(1) RT-PCR was used to detect the expression of PDCD4 in the laryngeal squamous carcinoma and adjacent non-cancerous tissues and discussed their associations between PDCD4 and tumor biological characters.(2) TaqMan? miRNA assays was employed to detect the expression of miR-21 in the laryngeal squamous carcinoma;【Result】:(1) By RT-PCR test , the down-regulated rate of PDCD4 mRNA in the laryngeal squamous carcinoma were 86.8%(P<0.05). Our study indicated that the expression of PDCD4 has a close relationship with proceeding of the laryngeal squamous carcinoma. Expression of PDCD4 mRNA was highly correlated with tumor differentiation,the TNM stage and lymph node metastasis(P<0.05). On the other hand, its expression was no significant different in the clinical type(P>0.05). In addition, experimental results had convincingly demonstrated that low expression of PDCD4 mRNA was highly associated with decreased tumor differentiation,the TNM stage and lymph node metastasis. while the lower expressions of PDCD4 mRNA in tumor tissues with lymphatica metastasis is higher than these with out lymphatica.(2) By matched samples Wilcoxon signed-rank test,finding datas of miR-21 had statistic meaning(P<0.05),and the expression of miR-21 was up-regulated which had significantly different in the laryngeal squamous carcinoma.【Conclusion】:The expressions of miR-21 and PDCD4 mRNA was a significantly Negative correlation in the laryngeal squamous carcinoma. The Abnormal expression of the miR-21 and PDCD4 mRNA might have relationships with the formation of the laryngeal squamous carcinoma. miR-21 might inhibit the expression of PDCD4,to reduce the ability of PDCD4 to inhibit tumor cell growth, thus, contributed to tumorigenesis and development.