| The present study was to identify and quantitate differentially expressed proteins in laryngeal squamous cell carcinoma (LSCC) tissues with or without lymph node metastasis, and to explore transcriptional factors and regulation networks associated with the process. Tissue specimens from20patients with LSCC, including10cases of LSCC without metastasis LSCC (NO) and10cases of LSCC with metastasis LSCC (N+). Among the643unique proteins identified by using iTRAQ labeling and quantitative proteomic technology,389proteins showed an abundance change of in LSCC (N+) as compared to LSCC (NO),244proteins showed overexpressed (more than1.2-fold) and145proteins underexpressed (less than0.8-fold). Cytoskeleton remodeling, cell adhesion and immune response activation were found to be the main processes in LSCC metastasis. The construction of transcription regulation networks identified key transcription regulators for lymph node metastasis of LSCC, including Spl, c-myc, and p53, which may affect LSCC metastasis through epithelial-mesenchymal transition. Furthermore, our results suggest that ubiquitination may be a critical factor in the networks. The present study provides insights into transcriptional factors and regulation networks involved in LSCC metastasis, which may lead to new strategies for treatment of LSCC metastasis. The present study was to identify and quantitate differentially expressed proteins in Hypopharyngeal Squamous Cell Carcinoma (HSCC) tissues with lymph node metastasis, and to explore transcriptional factors and regulation networks associated with the process. Tissue specimens from10patients with Hypopharyngeal Squamous Cell Carcinoma with lymph node metastasis, including the primary site of HSCC, adjacent tissues and lymph node of HSCC. Among the643unique proteins identified by using iTRAQ labeling and quantitative proteomic technology,382proteins showed an abundance change of in the primary site of HSCC as compared to adjacent tissues of HSCC.177proteins showed overexpressed (more than1.2-fold) and205proteins underexpressed (less than0.8-fold).459proteins showed an abundance change of in the lymph node of HSCC as compared to adjacent tissues of HSCC.241proteins showed overexpressed (more than1.2-fold) and218proteins underexpressed (less than0.8-fold). Cytoskeleton remodeling, cell adhesion and Blood coagulation were found to be the main processes in HSCC metastasis. The construction of transcription regulation networks identified key transcription regulators for lymph node metastasis of HSCC, including Sp1, c-myc, and HNF4-alpha, which may affect HSCC metastasis through epithelial-mesenchymal transition. Ubiquitination may not be a critical factor in the networks. Furthermore, our results suggest that the different immune responses between tumor and host immune system may cause the different metastatic capabilities between laryngeal and hypopharyngeal carcinoma. The present study provides insights into transcriptional factors and regulation networks involved in HSCC metastasis, which may lead to new strategies for treatment of HSCC metastasis. Objective:To comfirm the proteomics results and investigate the expression and clinical significance of fibronectin, ubiquitin and YB1in laryngeal squamous cell carcinoma.Methods:To use immunohistochemistry technology to detect fibronectin, ubiquitin and YB1expressions in20cases laryngeal carcinoma without metastasis and20cases laryngeal carcinoma with metastasis in paraffin tissue samples.Results:The fibronectin and ubiquitin proteins in laryngeal carcinoma with metastasis were over-expressed; YB1prtein in laryngeal squamous cell carcinoma with metastasis was low-expressed.Conclusion:These results demonstrated the proteomics researches in laryngeal squamous cell carcinoma with the cervical lymph node metastasis, suggesting that they may be play an important role in the metastasis and may be correlated with EMT.
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