The Research Of The Interaction With Histone Deacetylase 4 And Vascular Endothelial Growth Factor Receptor-1 On Adhesion, Invasion And Metastasis In Human Cancer Cell Line HepG2 In Vitro

Part I Expressions of HDAC4 and VEGFR-1 in human cancer cell line HepG2 in vitroObjective: To investigate the expressions of HDAC4 and VEGFR-1 in human cancer cell line HepG2.Methods:Human Carcinoma cell line HepG2 was cultured in vitro, Western Blotting and immunocytochemistry were used to examine the expression of HDAC4 and VEGFR-1.Result: The immunocytochemistry showed that protein HDAC4 expressed in brown granulars, which is mainly located in cell nuclei, and partly in intracytoplasm; protein VEGFR-1 expressed in brown granulars, which is located in intracytoplasm. Western-blotting showed that HDAC4 and VEGFR-1 expressed in human cancer cell line HepG2 with bands.Conclusion:HDAC4 and VEGFR-1 expressed in human cancer cell line HepG2.Part II the interaction with HDAC4 and VEGFR-1Objective: To explore the interaction between HDAC4 and VEGFR-1 after human Carcinoma cell line HepG2 cultured in vitro was treated with SPB.Methods: Human Carcinoma cell line HepG2 treated by SPB was cultured in vitro, Western Blotting and immunocytochemistry were used to examine the interaction between HDAC4 and VEGFR-1Result: Comparing with the control, protein HDAC4 and VEGFR-1 in brown granulars of intracytoplasm and cell nuclei became weaken after Carcinoma cell line HepG2 was treated with SPB for 24h; the down regulation expression of HDAC4 and VEGFR-1 were observed by Western Blotting after Carcinoma cell line HepG2 was treated with SPB,and it is showed in a time- and a dose-dependent manners.Conclusion:The expression of HDAC4 and VEGFR-1 was inhibited after Carcinoma cell line HepG2 was treated with SPB, which indicates that HDACI perhaps inhibit the expression of HDAC4 and VEGFR-1.PartⅢthe effects of HDACI on invasion, metastasis and adhesion in human cancer cell line HepG2 in vitroObjective: To observe the effects of HDACI on invasion, metastasis and adhesion in human cancer cell line HepG2 in vitro.Methods:The adhesion, invasion and metastasis was observed through transwell chambers, MTT method was used to analyze the adherence ability of human Carcinoma cell line HepG2.Result: Through the transwell chambers, the invasion cell numbers of the SPB-treated group was significantly less than that in control group (161.80±46.796,329.20±55.993 respectively, P<0.01 vs control), restraining ratio comes to 50.85%; the adhesion rate of HepG2 cells was significantly lower than in the controls (ρ<0.01 vscontrol).Conclusion:SPB inhibit the invasion, metastasis and adhesion of the human Carcinoma cell line HepG2. HDAC4 takes an important part in the adhesion, invasion and metastasis of the cells.