Expression And Clinical Significance Of P14ARF、MMP-2and TIMP-2in Cervical Cancer

Objectives:By detecting the expression of P14ARF、MMP-2and TIMP-2protein in cervical cancer and precancerous lesion、analyzing their relationship between clinical-pathologic and their expression, to investigate the role of P14ARF、 MMP-2and TIMP-2in the development of cervical cancer.Methods:Immunohistochemistry was used to measure the expression levels of P14ARF、MMP-2and TIMP-2protein in the cervix tissue specimens. The specimen contains20cases of normal cervical tissues,18cases of cervical intraepithelial neoplasia (CIN) and53cases of cervical carcinoma.Results:1. The expression of P14ARF can be seen in CIN and cervical carcinoma, but not in normal cervical tissues. The positive rate of P14ARF in CIN was61.1%(11/18),and81.1%(43/53) in cervical carcinoma.The positive rate of P14ARF in three groups has statistically significant difference (P<0.01).The positive rate of P14ARF in CIN I was20.0%(1/5), and76.9%in CIN Ⅱ-Ⅲ.There was statistically significant difference among them (P<0.05). The expression of P14ARF had relationship with the grade of differentiation and lymph node metastasis (P<0.05),but not correlated with type of organization, FIGO stage or myometrial invasion (P>0.05).2. The expression of MMP-2in normal cervical tissue、CIN and cervical carcinoma was10.0%(2/20)、33.3%(6/18)、73.6%(39/53) respectively, The positive rate of MMP-2in three groups has statistically significant difference (P<0.05),and arbitrary compared two groups, the result is the same(P<0.05). The expression of MMP-2had relationship with the grade of differentiation、lymph node metastasis and myometrial invasion (P<0.05),but not correlated with type of organization or FIGO stage (P>0.05).3. The expression of TIMP-2in normal cervical tissue、CIN and cervical carcinoma was15.0%(3/20)、44.4%(8/18)、52.8%(28/53) respectively, through statistical analysis, The expression of TIMP-2in normal cervical tissue has statistically significant difference with CIN and cervical carcinoma group respectively(P<0.05and P<0.01), there was no statistically significant difference between CIN and cervical cancer group (P>0.05).The expression of TIMP-2had relationship with lymph node metastasis and myometrial invasion (P<0.05),but not correlated with the grade of differentiation、 type of organization or FIGO stage (P>0.05).4. The Spearman rank correlation analysis found that positive expression of MMP-2and TIMP-2in cervical cancer tissue was negatively correlated (rs=-0.367, P<0.05).Conelusion:1. Positive expression of P14ARF in cervical cancer was significantly higher than normal cervical tissue and CIN group.In cervical carcinoma group, the expression of P14ARF had relationship with the grade of differentiation and lymph node metastasis.To a certain extent, positive expression of P14ARF reflected the malignant degree of cervical lesion and the clinical stage, p14ARF is expected to be a new indicator for predicting prognosis of cervical cancer.2. Positive expression of MMP-2and TIMP-2in cervical cancer was higher than normal cervical tissue and CIN group. MMP-2and TIMP-2may play an important role in the occurrence and development of cervical cancer.3. In cervical cancer, expressions of MMP-2and TIMP-2were related to the myometrial invasion and lymph node metastasis.In addition, the expression of MMP-2was also related to the grade of differentiation. MMP-2and TIMP-2may be associated with the invasion and metastasis of cervical cancer.4. In cervical cancer, positive expression of MMP-2and TIMP-2was negatively correlated. MMP-2/TIMP-2is expected to be one of the important indicators for predicting prognosis of cervical cancer.