| Objective To investigate the relationship between calcifying nanoparticles and calcium oxalate kidney stone formation.Method Kidney stones, renal pelvis urine and renal papilla calcified tissue spices were collected from 20 patients who were suffered from calcium oxalate kidney stones. Electron microscopy, Von kossa staining, X ray spectroscopy, Fourier transform infrared spectroscopy technologies were applied for identifying these calcifying nanoparticles. RPMI 1640 medium was as blank control.6 cases of patients with kidney tumor were in control group.2 The renal papilla calcified tissue in which the detection of calcifying nanoparticles were positive were searched for calcium salts site by light microscope.The ultrastructure of renal papilla calcified tissue were observed by electron microscopy.3 The morphology of calcifying nanoparticles which were cultured under free-serum was observed by electron microscopy in different periods.Results The calcifying nanoparticles positive rate in the spices of patients with calcium oxalate kidney stones was as follows:the core of kidney stone was 75%, surface was 60%, renal pelvis urine was 55%, and renal papillary calcifying tissue was 65%. A large number of calcifying nanoparticles were observed in renal papilla calcified tissue by scanning electron microscope, these particles adhered to the tubular epithelial cells. The particles which were similar to calcifying nanoparticles were also found in this tissue by transmission electron microscopy. The structure of calcifying nanoparticles under 40 weeks culture was similar to the Randall's plaque.Conclusion Calcifying nanoparticles are in close contact with the formation of calcium oxalate kidney stones:The appearance of these particles in calcified renal papillary tissue suggests that calcifying nanoparticles may play an important role in formation of calcified renal papilla-calcium oxalate kidney stones.The morphology of calcifying nanoparticles under free-serum culture is pleomorphic. |
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