The Inhibition Of Lewis Lung Carcinoma In Mice By Adenovirus Vector Mediated Endostatin

Tumor has become a kind of common disease in recent years. Malignant tumors have high mortality rate, short survival time. It is a serious hazard to people's health. Therefore, prevention and treatment of malignant tumor has become the hotspot of medical research.The current traditional treatments are unable to completely kill residual tumor cells, such as chemotherapy, surgery, radiotherapy, and this will lead to a low cure rate, a high recurrence rate and a hight mortality rate. With the improvement of the medical treatment level, prevention and treatment of tumor have made an important progress. However, how to improve the curative effect and reduce tumor recurrence?Further researches are still needed. So, the new research and development of therapies, especially anti-angiogenesis gene therapy, have got increasing attention. Cancer gene therapy is gradually becoming a new treatment method which has shown some magnificent prospects.Studies have shown that tumor growth is dependent on angiogenesis which needs new vessels to provide the nutrients and to carry away metabolic waste. Tumor can only grow to 1 ~ 2 mm without blood vessels. Thus, angiogenesis is one of the most important condition of tumor proliferation and metastasis. The method of anti-angiogenesis can effectively restrain tumor growth and metastasis, so as to treat tumor.In order to research the inhibition effect to Lewis lung carcinoma in mice, here we select adenovirus vector carrying endostatin gene, to make out theoretical and experimental basis for anti-angiogenesis gene therapy in clinic treatment.We classified experimental groups into tumor model group, AdCMV-LacZ group, AdCMV-endostatin group and prevent group. We measured tumor size, growth curve, and combined with pathological changes in tumor tissue we computed the microvessel density, apoptotic index and proliferation index of each group, using RT-PCR and Western Blot methods, to research expression of endostatin in mRNA and protein levels among tumor model group, AdCMV-LacZ group, AdCMV-endostatin group and prevent group.The results showed that AdCMV-endostatin can restrain the growth of Lewis lung carcinoma in mice. The mice tumor growth velocity in the AdCMV-endostatin group and prevent group is obviously slower than that of the tumor model group and AdCMV-LacZ group Compared with the tumor model group and AdCMV-LacZ group with the AdCMV-endostatin group and prevent group, the AdCMV-endostatin group and prevent group's microvessel density lessened sharply, the apoptotic index raised, the proliferation index lessened, and there are distinct differences. The expression of endostatin in mRNA levels increased clearly in the AdCMV-endostatin group and prevent group compared with the tumor model group and AdCMV-LacZ group. Western Blot proved that the expression of endostatin in protein levels also raised in AdCMV-endostatin group and prevent group.Conclusion: AdCMV-endostatin can inhibit the growth of Lewis lung carcinoma in mice, degrade the tumorous angiogenesis and promote the tumor cells'apoptosis.