Early Growth Response Factor-1 Decoy Oligonucleotide Down Regulate Matrix Metalloproteinases-9 Inhibit The Migration Of Original Rat Vascular Smooth Muscle Cells

ObjectiveBy detecting migration of rat aortic smooth muscle cell which was transfected Egr-1 decoy oligonucleotide expression changes of early growth response factor-1 and Matrix metalloproteinases-9 (MMP-9) was checked and the spread of migration was observered to approach the effect and mechanism of the decoy olligonucleotide of the early growth response factor-1 on the migration of vascular smooth muscle cells(SMC) of rats in vitro.MethodAfter the culture, serial subcultivation and identification of original arterial SMC form rats with tissue explants adherent method, Design and Synthesis of Egr-1 decoy to lure control oligonucleotides. Transfected Egr-1 decoy oligonucleotide (Egr-1 decoy ODN) on vascular smooth muscle cells. Packet the cultured cells into normal control group (normal cultured cells), decoy group (transfected Egr-1 decoy oligonucleotide) and the control group (Egr-1 transfection decoy oligonucleotide miscellaneous yards).The exression of egr-1 and MMP-9 which its downstream related gene was checked using immunohistochemical method at different time points and than by image analysised.The migration lenth of each group was obverved by scratch method. All data used SPSS 13.0 statistical software for statistical analysis, measurement data to the mean±standard deviation (x±S) said that the comparison group of single-factor analysis of variance, p<0.05 with statistical significance.ResultsImmunohistochemical identification of the cell, identified as high-purity cultured cells in vascular smooth muscle cells. For transfection of Egr-1 lured by immunohistochemical analysis at different time points expression of Egr-1 and mmp-9. The migration lenth of the decoy group (transfection Egr-1 decoy oligonucleotide)is much shorter than control group (normal cultured cells) and lured the control group (Egr-1 transfection decoy oligonucleotide miscellaneous yards)(p<0.05).There was no significant difference between control group and lured the acontrol group.The expression of Egr-1 and mmp-9 was lower in decoy group,had significant difference compared with control group and lured the control group.ConclusionTransfection the decoy oligonucleotide of egr-1 can inhibit the transfection of the arterial smcs of rat in vitro,reduction the exression of egr-1 and downstream gene MMP-9, Achieve the purpose of inhibiting cell transfection.