| Revascularization therapy has become one of the principal treatment of ischemic heart disease. Statins on multiple levels through a variety of ways achieve cardiovascular protective effects, including plaque stabilization, anti-inflammatory, antioxidant, etc. Studies have confirmed that statins on young rats with myocardial ischemia and reperfusion has a protective effect, mainly to reduce myocardial infarct size after ischemia and reperfusion and to improve hemodynamics, etc. But a lack of awareness of elderly subjects. On rat liver and kidney organs except in the heart of myocardial ischemia in the change process are still to be studied. This study focused on the study of statins in elderly myocardial ischemia reperfusion in rats, and further attention to the liver, renal function and possible mechanisms.1. Objective1.1. To observe the mortality rate, hemodynamics and myocardial infarct size of atorvastatin on myocardial ischemia reperfusion in aged rats.1.2. To observe the liver and kidney function of atorvastatin on myocardial ischemia reperfusion in aged rats.1.3. to analyse the possible mechanism of multiple organ function changes of atorvastatin on myocardial ischemia reperfusion in aged rats.2. Methods2.1. Wistar rats at 20 month old were given atorvastain(10,1mg/kg/d) for 4 month. Monitoring myocardial ischemia reperfusion groups mortality, hemodynamics, and myocardial infarct size.2.2. Observed liver function (GPT, GOT), renal function (Cr, BUN) of rats before and after reperfusion. Observation of the rats liver and kidney changes in pathology.2.3. The expression of eNOS was detected by western-bolt in rat heart, liver and kidney. The gene expression of eNOS-mRNA was detected by RT-PCR.3. Results3.1. In the course of myocardial ischemia-reperfusion, compared with the aged control group, the mortality rate of arrhythmia was significantly lower in statin group and young control rats (p<0.05). High-dose statin group mortality had no significant difference between the young group.30 minutes after ischemia, Rats LVSP,±dp/dtmax were significantly lower (p<0.05). After Reperfusion, High-dose and low dose statin group LVSP,±dp/dtmax, LVEDP has been restored. Postischemia, Compared with young control group, Aged control group, low dose statin group left ventricular systolic pressure,+dp/dtmax,-dp/dtmax were significantly decreased (p<0.01). LVDP increased significantly. High-dose statins were no significant differences (p>0.05). After reperfusion, Compared with the aged control group, the percentage of myocardial infarct size of High-dose statin group also significantly reduced than control group youth (p<0.05).3.2. After reperfusion, liver and kidney function significantly increased in Aged control rats (p<0.05). Liver function were also elevated in low-dose statin group (p<0.05). High-dose statin group and young control group compared with the previous indicators of liver and kidney function were no significant differences (p>0.05). In HE staining, Light microscope, liver cells and kidney cells in the aged control group were disordered and fibrosis.3.3. After reperfusion, compared with young and aged control group, eNOS synthesis and expression of eNOS-mRNA increased significantly in statin group (p<0.05). High-dose statins group increased more significantly than lower dose statin group (p<0.05).4. Conclusion 4.1. Atorvastatin reduces mortality in the process of myocardial ischemia reperfusion in aged rats.4.2. Atorvastatin can prevent hemodynamic deterioration in the process of myocardial ischemia reperfusion in aged rats, reduce the myocardial infarction area.4.3. After reperfusion in aged rats, Prone to liver and kidney dysfunction, have the protective effects of liver and kidney function on myocardial ischemia reperfusion in aged rats. The protective effect of multiple organ in both the heart, liver, kidney. This role was related with the increase of synthesis of eNOS and eNOS-mRNA. |
