| In recent years, Electrochemiluminescence (ECL) was applied to a variety of fields such as biosensors, immunoassays, and flow injection analysis, due to its low background noise, simplicity, inherent sensitivity, wide linear range and good selectivity. On the other hand, capillary electrophoresis (CE) has gradually became an important and powerful analytical tool in pharmaceutical analysis according to its small sample size, high sensitivity, rapidity, short analysis time, and low operational cost. ECL using tris(2,2-bipyridyl)ruthenium(Ⅱ) (Ru(bpy)32+) system has been extensively studied and the marriage of CE and ECL using Ru(bpy)32+has been studied for the determination of a variety of analytes. It has been widely used in various fields, such as pharmaceutical analysis, food analysis, bio-analysis, and the separation and analysis of other samples.This thesis was concentrated on the new development and improvement of CE-ECL separation and detection technique, and enlarging its application in new fileds. The obtained resulits were described below:1. Granisetron hydrochloride (GRN), is a selective 5-HT3 receptor antagonist which is effective in controlling vomiting and nausea resulting from cancer therapy associated with many cytotoxic chemotherapeutic agents by injection or oral administration of GRN. A new method for the determination of GRN in rat plasma was developed using electrochemiluminescence detection coupled with capillary electrophoresis (CE-ECL). The parameters that affected the detection and separation were optimized. Under the optimal conditions, the linear range for GRN was from 0.001 to 5μg/mL (r2=0.9993), and the detection limit was 0.16 ng/mL (S/N=3). The method was successfully applied for the determination of GRN in rat plasma. The relative standard deviations of ECL intensity and migration time were 2.3% and 1.0%, respectively (1.0μg/mL GRN, n=6). The recovery is from 95.28% to 96.20%.2. Propranolol hydrochlorid (PRO), has recently been introduced a drug asβ-adrenoceptor antagonist, for its extensively application in the therapy of hypertension, angina pectoris, cardiac arrhythmia, hypertrophic subaortic stenosis in clinical practice, and the International Olympic Committee has forbidden it in some games such as shooting and weightlifting. CE-ECL was applied for the determination of PRO in human urine. The parameters that affected the detection and separation were optimized. Under the optimal conditions, the linear range for PRO was from 0.003 to 2μg/mL (r2=0.9993), and the detection limit was 1.3 ng/mL (S/N=3). This method was applied to the study of pharmacokinetics of PRO successfully in human urine. The relative standard deviations of ECL intensity and migration time were 2.6% and 2.1%, respectively (1.0μg/mL PRO, n=6). The recovery is between 96.71% and 97.30%. The peak excretion rate was observed in urine during the 0.5-1 h after oral administration of 10 mg PRO tablet and the urinary excretion ratio of PRO was 13.6% within 12 h. The method is simple, rapid, economical, sensitive, and may make impact on the detection of doping agent in sports and possesses good application prospect.3. Cetirizine Hydrochloride (CTZ) is a piperazine derivative and active metabolite of hydroxyzine, which is widely used in the treatment of seasonal allergic rhinitis and perennial allergic rhinitis as well as chronic urticaria and pruritus. A simple, quick, sensitive and selective method was developed for the determination of CTZ in human plasma by utilizing CE-ECL. The parameters that affected detection and separation of CTZ were optimized. Under the optimized conditions, the linear range for CTZ concentration was from 0.5 to 50μg/mL (r2=0.9966) with a detection limit of 104 ng/mL (3a). The relative standard deviations of ECL intensity and migration time were 1.9% and 1.3%, respectively (5.0μg/mL CTZ, n=6). The recovery is from 96.04% to 97.10%. |
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