| Objective To observe the effect of inducible nitric oxide synthase inhibitor SMT on portal hypertension esophageal varices in rats.Methods 60 healthy male SD rats were randomly divided into five groups, sham operation group, model group, low dose SMT treatment group, middle dose SMT treatment group and high dose SMT treatment group. The portal vein and left adrenal vein were not ligated in sham-operated group. The other groups were given a two-stage ligation of the portal vein plus ligation of the left adrenal vein. Sham-operation group and model group were given intraperitoneal injection of normal saline. Other three groups were given intraperitoneal injection with different concentrations SMT. On 21 days after surgery, portal vein pressure was measured; The total nitric oxide synthase (TNOS) activity, inducible nitric oxide synthase (iNOS) activity and NO content in portal vein were determind; The numbers and area of esophageal submucosal vessels which were marked by CD34 were measured by image analytical apparatus.Results The model group had a significant increase in the TNOS activity, the iNOS activity, the NO content of portal blood, as well as in the number, the average cross-sectional area, total cross-sectional area of esophageal submucosal vessels comparied with the sham-operation group (P<0.01). Whereas the middle-dose SMT treatment group and high-dose SMT treatment group had a significant decrease in the above parameters comparied with the model group (P<0.05).Conclusion:1. NO play an important role in the pathogenesis of esophageal varices.2. NO is mainly generated by iNOS in the portal hypertension esophageal varices induced by ligating partially portal vein.3. SMT have some protective effect on esophageal varices in rats with portal hypertension.
|
PDF Full Text Request