The Study On Developmental Neurotoxicity Of Fenvalerate In Vitro

Objective To investigate the effect of fenvalerate on mNSCs migration and differentiation and the neurite growth in hippocampus in vitro.Methods Dose-dependent cytotoxicity of fenvalerate on mNSC in differentiating and the hippocampus cells at 48 h was deceted by MTT assay, LDH assay and PI staining (only LDH assay was used to assay the cytotoxicity of hippocampus cells). Then the immunocytochemistry staining was used to determine the migration and differentiation of mNSC, and the neurite growth in hippocampus neurons.Results With the increase of fenvalerate dose, it strengthened cytotoxicity of mNSC in differentiation and the hippocampus cells (â‘ mNSC in differentiating: MTT assay, r=-0.831; LDH assay, r=0.752;PI staining, r=0.835;â‘¡the hippocampus cells : LDH assay, r=0.52. all P < 0.01). Under the condition of no significant cytotoxicity, FEN inhibited the migration and differentiation, especially the astrocytic differentiation of mNSC. In addition, neurons were more vulnerable to FEN than astroglias and it decreased the length and number of neurite in a dose-dependent manner.Conclusions The results indicated that fenvalerate could induce cytotoxicity on mNSC in differentiating and the hippocampus cells. FEN inhibited the mNSCs differentiate to astroglias in the differentiation stage. And then, it disrupted and/or inhibited neurite growth in neurodevelopment. Therefore, these two points might play critical roles in FEN's developmental neurotoxicity.