Study On Mrna Expression Of TNIP1 Gene In Peripheral Blood Mononuclear Cells Of Sle Patients

Background Systemic lupus erythematosus (SLE) is a clinical complex autoimmune disease, which can affect the kidneys, lungs, heart, blood and other organs and systems, with a variety of autoantibodies appeared in serum. The pathogenesis of SLE has not been entirely elucidated. Its pathogenesis is associated with heredity, environment, immunization, viral infections, drugs and hormones and other factors. Generally the genetically susceptible individuals are prone to develop SLE when some pathological changes occurred under the influence of many factors, such as immune function abnormalities, autoantibody production, immune complex formation and deposition in the organization. However,genetic factors play an important role in the pathogenesis of SLE. A number of susceptibility genes in SLE have been found by genome-wide scanning and candidate gene approach. In recent years, genome-wide association study (GWAS) has become a highly efficient approach to search for the susceptibility genes of polygenic diseases. Many new susceptibility genes and loci of SLE have been identified in different ethnic groups. The two single nucleotide polymorphisms (Single nucleotide polymorphism, SNP) rs7708392 and rs10036748 in TNIP1 gene (P = 4.5×10-7; P = 1.67×10-9) are reported in Europe and Chinese Han population, respectively, significantly associated with SLE. As tumor necrosis factor-α-induced protein 3 (TNFAIP3) of interacting proteins, TNIP1 is synergistic with TNFAIP3 gene to inhibit NF-κB activation and TNF-mediated apoptosis. In this study, we used the real-time PCR to analyze TNIP1 mRNA in the cases and normal controls in order to explore the role of TNIP1 in the pathogenesis of SLE. Objectives To explore the mRNA expression of TNIP1 gene in SLE patients'peripheral blood mononuclear cells (PBMCs), as well as its relationship with the SLE Disease Activity Index (SLEDAI) and SNP rs10036748.Methods we collected 61 patients with SLE and 66 normal controls. The mRNA was extracted from the peripheral blood mononuclear cells (PBMC) and then reverse transcription it for cDNA. We used the ABI Prism 7900-based high-throughput fluorescence quantitative-PCR to detect the expression of TNIP1 mRNA in PBMCs. The SNP rs10036748 was genotyped by application of Sequenom MassArray technology, then using the SPSS13.0 software for statistical analysis.Results The mRNAs expression level of TNIP1 gene in the SLE patients was lower than the healthy controls (P = 0.0004). No correlation was found between mRNA expression level of TNIP1 gene and SLEDAI scores or SNP rs10036748 (P > 0.05).Conclusion It suggestes that the TNIP1 gene may play an important role in the pathogenesis of SLE.