The Effect Of RNA Interference Target Livin Gene In The SPC-A-1 Cell Transplanted Tumor In Nude Mice

BackgroundLung cancer is one of the most common malignancies worldwide ,Recent studies have suggested that Livin is highly expressed in lung cancer. It may be involved in tumorigenesis and development of lung cancer.In this study,we used RNA interference technology to silence Livin expression,then observed the effects of the proliferation of adenocarcinoma of lung cell SPC-A1, and then we established the tumor-bearing nude mice model of SPC-A-1, explored the possible mechanism in vivo.Objective:To explore the effect of proliferation of transplantation tumor induced by SPC-A1 in vivo after silencing Livin gene by RNA interference technology.Methods:Livin shRNA was transfected into SPC-A-1 cells by lentivector, then three different nude mice models were established by inoculating different disposal SPC-A1 cells into three nude mice groups subcutaneously,one group were inoculated with blank SPC-A-1 cells(the blank control group), one were inoculated with cells transfected with non-transfected lentivirus vectors ( the negative group), another were inoculated with cells with lentivirus-delivered LivinshRNA(the experimental group). Following on, the formation time,the formation rate, the tumor volume and weight of these tumors were measured in different time points;the curve of tumor growth were described; and the inhibition rate was calculated. Livin gene expression in these tumor tissues were detect by RT-PCR and Inmunohistochemistry assay. Cell apoptosis of tumor tissues was detected by TUNEL assay.Results:The study showed a slower tumor growth , a smaller tumor volume and a lighter tumor weight in experimental group than the blank and negative groups (P<0.05). The volume inhibition rate was ( 59.5±3.4 ) %(P<0.05), and the weight inhibition rate was (71.1±5.6)%(P<0.01). LivinаmRNA and LivinβmRNA expression in the experimental group were about (37.2±1.6)%and (29.4±1.1)% respectively by RT-PCR assay, which were significantly lower than two control groups (P<0.05). Livin protein expression level was significantly lower than the blank groupand the negative group too[(15.3±2.8)% versu(s51.3±2.1)% ,(52.5±2.5)% (P<0.05)]. The apoptosis rate by TUNEL assay in the experimental group was significantly higher than these two control groups as well [(35.4±3.2)% versus(5.4±1.3)%and(8.6±1.5)%(P<0.05)].Conclusions:The lentivirus-delivered LivinshRNA can inhibite the proliferation of transplantation tumor of lung carcinoma effectively,Livin may be a potent target of gene therapy in lung cancer.